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A kind of cultivation method for improving the synthetic phycoerythrin content of Porphyridum algae

The technology of a phycoerythrin and a culture method, which is applied in the field of marine microorganisms, can solve the problems of insufficient phycoerythrin content and low biomass content, and achieve the effects of easy control, simple conditions and reduction of light intensity.

Active Publication Date: 2021-03-30
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing research, in addition to extracting and purifying phycoerythrin, how to increase the source of raw materials is becoming more and more important. However, due to the limitation of production capacity, the method of cultivating Porphyridium is usually achieved by monoculture methods such as adding nutrients and maintaining low temperature and low light conditions. The purpose of increasing the content of phycoerythrin, this method can increase the content of phycoerythrin to a certain extent, but the common problem is that the biomass content is low and the content of phycoerythrin is not high enough

Method used

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  • A kind of cultivation method for improving the synthetic phycoerythrin content of Porphyridum algae
  • A kind of cultivation method for improving the synthetic phycoerythrin content of Porphyridum algae
  • A kind of cultivation method for improving the synthetic phycoerythrin content of Porphyridum algae

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Embodiment 1

[0025] The cultivation method for increasing the phycoerythrin content of Porphyridium purpureum by segmental control of temperature and light intensity in this embodiment adopts Porphyridium purpureum CoE1 for cultivation, comprising the following steps:

[0026] (0) Normal culture: the conditions are temperature 25°C, light intensity 165μmol / m 2 s. The sterile air ventilation rate is 1L / min, inoculate the Porphyridium algae liquid normally cultivated to the end of the logarithmic phase of growth into the artificial seawater medium, and control the initial OD 680 The value is 0.4 to 0.6.

[0027] Among them, the formula of artificial seawater culture medium is as follows:

[0028] Table 1 Artificial Seawater Medium (ASW)

[0029] Table 1 ASW medium

[0030]

[0031] ① Trace metal salt solution mother solution (mg / L): ZnCl 2 4.0,H 3 BO 3 60.0, CoCl 2 2H 2 O 4.0, CuCl 2 2H 2 O 4.0, MnCl 2 4H 2 O 40.0, (NH 4 ) 6 Mo 7 o 24 4H 2 O 37.0.

[0032] ② Chelated i...

Embodiment 2

[0038] The difference between embodiment 2 and embodiment 1 is:

[0039] (1) Control the temperature at 25°C and the light intensity at 165μmol / m 2 Cultivate for 2 days under s;

[0040] (2) Control the temperature at 20°C and the light intensity at 110μmol / m 2 Cultivate for 8 days under s;

[0041] (3) Control the temperature at 15°C and the light intensity at 55μmol / m 2 2 days under s is the end of the culture. The content of phycoerythrin synthesized by Porphyrococcus was improved by controlling the temperature and light intensity in sections and changing the culture conditions.

[0042]After culturing in this embodiment, the phycoerythrin content can reach 205.3 mg / L, and the biomass content can reach 8.4 g / L.

Embodiment 3

[0044] The difference between embodiment 3 and embodiment 1 is:

[0045] (1) Control the temperature at 27°C and the light intensity at 185μmol / m 2 Cultivate for 3 days under s;

[0046] (2) Control the temperature at 22°C and the light intensity at 130μmol / m 2 s for 9 days;

[0047] (3) Control the temperature at 18°C ​​and the light intensity at 75μmol / m 2 3 days under s is the end of the culture. The content of phycoerythrin synthesized by Porphyrococcus was improved by controlling the temperature and light intensity in sections and changing the culture conditions.

[0048] After culturing in this embodiment, the content of phycoerythrin can reach 189.2 mg / L, and the content of biomass can reach 7.7 g / L.

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Abstract

The invention discloses a culture method for increasing the content of phycoerythrin synthesized by porphyridium purpureum. The method aims at inducing the porphyridium purpureum cells to synthesize more phycoerythrin by changing environmental conditions and lowering the temperature and the light intensity. The phycoerythrin content of the porphyridium purpureum cultured by the method is 109.2-205.3 mg / L, the biomass is 5.3-7.7 g / L, and the porphyridium purpureum has important value in the fields of medicines, foods and the like.

Description

technical field [0001] The invention belongs to the technical field of marine microorganisms, and in particular relates to a cultivation method for increasing the content of phycoerythrin synthesized by Porphyrococcus. Background technique [0002] Phycoerythrin is a food-grade pigment with excellent water solubility. It mainly exists in the outermost end of phycobilisomes. Chromophores and apoproteins are covalently combined through thioether bonds. Phycoerythrin has good fluorescence intensity, anti-oxidation, free radical scavenging, high chroma and other conditions, and is widely used in food, cosmetics, pharmaceuticals and other industries. It has a wide range of uses, and phycoerythrin can also be used as a fluorescent marker in molecular biology, clinical medicine and other fields. For example, it can be used in photodynamic therapy of tumors, because of its safe and natural characteristics as a very A good photosensitizer has great potential. [0003] Porphyridium ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 曾宪海徐远超吴升山焦凯琳孙勇唐兴林鹿
Owner XIAMEN UNIV