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Pseudomonas inhibiting composition and application thereof in preparation of medicine for preventing and treating pseudosciaena crocea visceral white spot disease

A technology for Pseudomonas spp. and visceral white spot disease, which is applied in the field of large yellow croaker culture and can solve the problems of undiscovered inhibition of Gram-negative bacteria and the like

Inactive Publication Date: 2019-08-27
FUJIAN MINDONG AQUATIC PROD RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the different structure of the bacterial cell wall, according to current findings, nisin only has a significant inhibitory effect on Gram-positive bacteria, and has not yet been found to inhibit Gram-negative bacteria.
Therefore, it is not currently possible to use nisin alone to inhibit Pseudomonas (Gram-negative bacteria)

Method used

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  • Pseudomonas inhibiting composition and application thereof in preparation of medicine for preventing and treating pseudosciaena crocea visceral white spot disease
  • Pseudomonas inhibiting composition and application thereof in preparation of medicine for preventing and treating pseudosciaena crocea visceral white spot disease
  • Pseudomonas inhibiting composition and application thereof in preparation of medicine for preventing and treating pseudosciaena crocea visceral white spot disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The composition for inhibiting Pseudomonas is mainly a mixed aqueous solution of nisin Z and polymyxin B, wherein the mass ratio of nisin Z to polymyxin B is 500:1, and the concentration of nisin Z is 250 μg / ml.

[0017] During the outbreak of large yellow croaker visceral white spot disease, pick the large yellow croaker that can detect Pseudomonas in vitro, after mixing every kilogram of large yellow croaker with 40 ml of the described Pseudomonas inhibitory composition for 24 hours, Pseudomonas was not detected in vitro in large yellow croaker, and Pseudomonas was still not detected in vitro after 1 week, and no white spots were found in the viscera of large yellow croaker after dissection.

Embodiment 2

[0019] The Pseudomonas inhibitory composition is mainly a mixed aqueous solution of nisin Z and polymyxin B, wherein the mass ratio of nisin Z to polymyxin B is 1000:1, and the concentration of nisin Z is 500 μg / ml.

[0020] During the outbreak of large yellow croaker visceral white spot disease, pick the large yellow croaker that can detect Pseudomonas in vitro, after mixing every kilogram of large yellow croaker with 50 ml of the described Pseudomonas inhibitory composition for 20 hours, Pseudomonas was not detected in vitro in large yellow croaker, and Pseudomonas was still not detected in vitro after 1 week, and no white spots were found in the viscera of large yellow croaker after dissection.

Embodiment 3

[0022] The Pseudomonas inhibitory composition is mainly a mixed aqueous solution of nisin Z and polymyxin B, wherein the mass ratio of nisin Z to polymyxin B is 2000:1, and the concentration of nisin Z is 1000 μg / ml.

[0023] During the outbreak of large yellow croaker visceral white spot disease, pick the large yellow croaker that can detect Pseudomonas in vitro, after mixing every kilogram of large yellow croaker with 45 ml of the described Pseudomonas inhibitory composition for 22 hours, Pseudomonas was not detected in vitro in large yellow croaker, and Pseudomonas was still not detected in vitro after 1 week, and no white spots were found in the viscera of large yellow croaker after dissection.

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Abstract

The invention relates to a Pseudomonas inhibiting composition and application thereof in the preparation of a medicine for preventing and treating pseudosciaena crocea visceral white spot disease. ThePseudomonas inhibiting composition is mainly a mixed aqueous solution of nisin Z and polymyxin B, wherein the mass ratio of nisin Z to polymyxin B (hereinafter referred to as PB) is (500 to 4000):1 and the concentration of nisinZ is 250 to 2000 mug / ml. The Pseudomonas inhibiting composition has obvious inhibiting effects on various Gram-negative bacteria, and particularly has obvious long-term inhibiting effect on the pseudosciaena crocea visceral white spot pathogenic bacteria, so as to effectively prevent and treat pseudosciaena crocea visceral white spot disease.

Description

technical field [0001] The invention relates to the field of large yellow croaker breeding, in particular to a composition for inhibiting Pseudomonas and its application in the preparation of medicines for preventing and treating visceral white spot disease of large yellow croaker. Background technique [0002] The large yellow croaker has fresh and tender meat and high nutritional value. It is one of the traditional "four sea fishes" in my country and the main economic fish in the coastal waters of our country. With the continuous expansion of the scale of large yellow croaker farming, the load on the farming environment is also increasing, and various diseases of large yellow croaker are becoming more and more frequent. White spot disease of large yellow croaker viscera is one of the more common diseases of large yellow croaker cultured in cages in recent years. The resulting high mortality of large yellow croaker has brought serious economic losses to large yellow croaker...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61P31/04A61K38/12
CPCA61K38/164A61K38/12A61P31/04
Inventor 徐春霞匡珍谢友佺迟海张芳芳黄慧珍
Owner FUJIAN MINDONG AQUATIC PROD RES INST
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