Application of flavonoid

A kind of technology of flavonoids, hydroxyflavonoid dihydrate

Inactive Publication Date: 2019-09-06
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF0 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these drugs are limited by factors such as lack of oral

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of flavonoid
  • Application of flavonoid
  • Application of flavonoid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Screening of inhibitors of PD-1 / PD-L1 protein interaction

[0030] ① Dilute the His-Trx-PD-1 fusion protein expressed by E. coli to 1 μg / mL with carbonate coating buffer, add 100 μL per well to a 96-well microtiter plate, and place at 4 °C overnight. Wash 3 times with 1×PBST, block with 5% skimmed milk powder, 300 μL per well, block at 37°C for 2hrs.

[0031] ② Wash 3 times with 1×PBST. At the same time, dilute GST and GST-PD-L1 proteins to 2 μg / mL with 1×PBS, add 0.2 μL of DMSO or small molecule compound (final concentration 5 μM), mix well, add 100 μL to each well of a 96-well plate, and combine at 37 °C 2hrs. Wash 3 times with 1×PBST, add 100 μL of GST antibody (rabbit polyclonal antibody) diluted in 1×PBS to each well, and bind at 37°C for 2hrs. Wash 3 times with 1×PBST, add 100 μL of HRP-labeled goat anti-rabbit antibody diluted in 1×PBS to each well, bind at 37°C for 0.5hrs.

[0032] ③Wash 5 times with 1×PBST, add 100 μL TMB substrate chromogenic solution ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an application of a flavonoid compound, the flavonoid compound is 3, 3', 4', 5, 7-pentahydroxyflavone dihydrate, the ELISA and protein fluorescence quenching experiments provethat the compound can effectively inhibit interaction of PD-1/PD-L1 proteins, and has a strong affinity for PD-L1 protein. Cell clone number formation and ELISA detection of IL-2 secretion assay prove that the compound can effectively enhance the tumor killing activity of T lymphocyte Jurkat cells against MDA-MB-231 and NCI-H460 tumor cells as well as the expression level of IL-2, and then reverse T cellular immunosuppression. The research results of the application provide a drug for tumor immunotherapy targeting the PD-1/PD-L1 immunological test site.

Description

technical field [0001] The invention relates to the application of a small molecular compound, especially the application of a flavonoid compound. Background technique [0002] T cells are important immune cells and play an important role in the process of anti-tumor immunotherapy. Activation of T cells is a highly regulated process that requires, in addition to the first signal transduced by the T cell receptor (TCR) (TCR binds to the MHC-antigen peptide), co-signaling molecules (co-stimulatory and co-inhibitory) ) to promote or inhibit T cell responses, wherein the second signal is the key to eliciting T cell antigen-specific responses. Co-signaling molecule receptor proteins include molecules such as CD28, PD-1, CTLA-4, ICOS, BTLA, LAG3, TIM3, and OX40, among which CD28, OX40, and ICOS are used to present co-stimulatory signals to activate T cell immune responses, PD-1, CTLA-4, BTLA, LAG3, and TIM3 receptor proteins are used to present co-inhibitory signals to suppress ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K31/352A61P35/00
CPCA61K31/352A61P35/00
Inventor 刁爱坡景磊李玉银
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap