Pharmaceutical applications of daphnetin in improvement of aging-related cognitive decline

A technology of daphnetin and drugs, applied in drug combinations, active ingredients of heterocyclic compounds, nervous system diseases, etc., can solve the problems of lack of effective drugs for the prevention and treatment of Alzheimer's disease

Inactive Publication Date: 2019-09-20
XI AN JIAOTONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From a global perspective, the prevention and treatment of Alzheimer's disease lacks effective therapeutic drugs, and it is urgent to find effective prevention drugs

Method used

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  • Pharmaceutical applications of daphnetin in improvement of aging-related cognitive decline
  • Pharmaceutical applications of daphnetin in improvement of aging-related cognitive decline
  • Pharmaceutical applications of daphnetin in improvement of aging-related cognitive decline

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 mouse water maze experiment

[0026] The Alzheimer's disease model mice were administered daphnetin for 9 months, and the mice in the corresponding experimental group were also given distilled water or daphnetin, and then the water maze experiment was performed.

[0027] The Morris water maze test is a classic method to test the spatial learning and memory ability of mice. The test method is as follows: it is carried out in a circular swimming pool with a diameter of 120cm. The swimming pool is divided into four quadrants: I, II, III and IV. A small round platform with a diameter of 7.5cm is placed in the center of the fourth quadrant, and the round platform is submerged below the water surface. 1cm. Four different-shaped markers were placed on the curtain brackets around the swimming pool for the four quadrants as visual cues. During the whole experiment, the water temperature was kept at 25°C, the surroundings were kept quiet, and the swimming pool was ...

Embodiment 2

[0030]Example 2 Detection of soluble β-amyloid (β-amyloid, Aβ) in brain tissue

[0031] One week after the end of the water maze experiment in Example 1, the mice were sacrificed, and the cerebral cortex tissue of the mice was taken out to detect soluble β-amyloid (β-amyloid, Aβ) in the brain tissue.

[0032] Aβ plaque is a significant pathological feature of Alzheimer's disease. In the Aβ pathway, Aβ 40 and Aβ 42 Most common; thus targeting soluble Aβ in the cerebral cortex 40 and Aβ 42 The content is detected, and the inspection method used is the ELISA method.

[0033] First, use (5mol / L guanidine hydrochloride / 50mmol Tris HCl) guanidinium buffer to homogenate the cortex tissue, centrifuge the tissue homogenate at 4°C, 10000g for 10 minutes, and take the supernatant for measurement. Dissolve 50 μL of known human Aβ 40 or Aβ 42 Standard products and homogenate samples of brain tissue to be tested were added to a 96-well plate pre-coated with Aβ antibody, and incubated ...

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Abstract

The invention relates to pharmaceutical applications of daphnetin in improvement of aging-related cognitive decline, and specifically discloses an application of daphnetin in preparation of drugs for preventing or treating diseases caused by increase of amyloid beta-proteins in a brain, and an application of daphnetin as an inhibitor of the amyloid beta-proteins in the brain. The invention also discloses an application of daphnetin in preparation of drugs for preventing or treating cognitive decline, namely Alzheimer's disease and Parkinson's disease.

Description

technical field [0001] The invention relates to the field of medicines, in particular to the application of daphnetin in the preparation of medicines for improving cognitive ability and preventing and treating senile dementia. Background technique [0002] Daphnetin, also known as zushimajialin, chemically named 7,8-dihydroxycoumarin (7,8-dihydroxycoumarin), is one of the natural medicines independently developed in my country. Daphnein is an active ingredient extracted from Daphne Korean Nakai, a plant of the genus Daphne, and is a representative monomer component of coumarin compounds. At present, daphnetin is clinically used for the treatment of thromboangiitis obliterans and other occlusive vascular diseases and adjuvant treatment of coronary heart disease. [0003] Recently, the inventor's research proved for the first time that daphnetin can improve the cognitive function of mice by reducing the level of β-amyloid (Aβ) in the early brain pathology of Alzheimer's disea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/37A61P25/28
CPCA61K31/37A61P25/28
Inventor 龙建纲高佩佩王珍祁红斌熊跃马庆庆梁冰刘健康
Owner XI AN JIAOTONG UNIV
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