A kind of preparation method and application of tussah silk fibroin nanoparticles

A nanoparticle and tussah silk fibroin protein technology, which is applied in the field of preparation of tussah silk fibroin nanoparticles, can solve the problems of complicated nanoparticle preparation process and increased use of organic solvents, and achieve good anti-inflammatory effect in vitro, good application potential, particle size The effect of uniform diameter distribution
CN110279674BActive Publication Date: 2021-11-02SOUTHWEST UNIVERSITY
0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTHWEST UNIVERSITY
Publication Date
2021-11-02

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to the technical field of nano drug carriers, and specifically discloses a preparation method and application of tussah silk fibroin nanoparticles. Turbid liquid, ultrasonic, centrifugal separation, freeze-drying and other operations. The present invention uses natural tussah silkworm cocoons with excellent biocompatibility as raw materials to ensure the biosafety performance of nanocarriers, and the whole preparation method is simple and quick, and tussah silk nano-particles containing RGD peptide natural targeting ligands are prepared to avoid It avoids the severe environment and the toxic and side effects caused by the residue of organic solvents in the modification process of foreign ligands, and at the same time has a good function of targeting macrophages, the particle size distribution is relatively uniform, and it has high drug loading and encapsulation. It has a good application potential in the treatment of ulcerative colitis.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of nano drug carriers, in particular to a preparation method and application of tussah silk fibroin nanoparticles. Background technique

[0002] Tussah silk fibroin (Antheraea pernyi silk fibroin, ApSF) protein is a natural polymer, which is only composed of H chains, according to the gene sequence of tussah silk fibroin (Sezutsu H, Yukuhiro K. Dynamic arrangement within the Antheraea pernyi silk The fibroin gene is associated with four types of repetitive units. J.Mol.Bio.2000; 51(4):329-338.) The amino acid sequence of the tussah silk fibroin protein deduced is composed of 2639 amino acid residues with a size of about 216kD composition. Alanine, glycine and serine are the three main amino acids constituting tussah silk fibroin, accounting for 43.09%, 27.26% and 11.25% of the total amino acids respectively. The head of tussah silk fibroin is the H chain, which consists of a residue sequence of 155 amino a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More