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A kind of preparation method and application of tussah silk fibroin nanoparticles

A nanoparticle and tussah silk fibroin protein technology, which is applied in the field of preparation of tussah silk fibroin nanoparticles, can solve the problems of complicated nanoparticle preparation process and increased use of organic solvents, and achieve good anti-inflammatory effect in vitro, good application potential, particle size The effect of uniform diameter distribution

Active Publication Date: 2021-11-02
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the modified targeting molecule is highly oriented, it complicates the preparation of nanoparticles and again increases the use of organic solvents

Method used

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  • A kind of preparation method and application of tussah silk fibroin nanoparticles
  • A kind of preparation method and application of tussah silk fibroin nanoparticles
  • A kind of preparation method and application of tussah silk fibroin nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A preparation method of tussah silk fibroin nanoparticles is characterized in that it comprises the following steps:

[0031] 1) Add softening liquid to the tussah cocoon and spread over the tussah cocoon, boil it twice for 30 minutes each time, then fully wash it with deionized water, and boil it with deionized water for 30 minutes to obtain the degummed and softened tussah silkworm Silk fibroin; wherein, sodium carbonate and sodium lauryl sulfate are included in the softening solution, and the concentrations of the sodium carbonate and sodium lauryl sulfate are 0.5% and 0.01% respectively, and the rest is water; and Compared with silkworm cocoons, tussah silkworm cocoons are more hydrophobic and harder in texture. Traditional weak alkali degumming methods cannot be used for degumming well, so sodium lauryl sulfate is added as a saponifier during the degumming process to soften the tussah cocoons. Silkworm cocoons, making it easier to degumming and facilitate the next ...

Embodiment 2

[0039] In this example, the tussah silk nano-medicine with its own targeting function was prepared by using the preparation method described in Example 1, which has a good targeting effect on macrophages. The tussah silk nano-particles The preparation method is widely used in the preparation of medicines for the treatment of ulcerative colitis. Specifically, the resveratrol-coated tussah silk nanomedicine with its own targeting function has a good anti-inflammatory effect in vitro, and is effective in the treatment of ulcerative colitis. It has great application potential.

Embodiment 3

[0041] A preparation method of tussah silk fibroin nanoparticles is characterized in that it comprises the following steps:

[0042] 1) Add softening liquid to the tussah cocoon and spread over the tussah cocoon, boil it twice for 30 minutes each time, then fully wash it with deionized water, and boil it with deionized water for 30 minutes to obtain the degummed and softened tussah silkworm Silk fibroin; wherein the softening solution includes sodium carbonate and sodium lauryl sulfate, and the concentrations of sodium carbonate and sodium lauryl sulfate are 0.5% and 0.01% respectively;

[0043] 2) Dry the degummed and softened tussah fibroin in an oven, then completely dissolve it in a lithium thiocyanate solution with a concentration of 13 mol / L, and dialyze it in an ice-water bath for 3 days to obtain the regenerated tussah silk fibroin; Since tussah silkworm cocoons contain a large number of microcrystals formed by β-sheet stacks formed by alanine repeat sequences, it is d...

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Abstract

The invention relates to the technical field of nano drug carriers, and specifically discloses a preparation method and application of tussah silk fibroin nanoparticles. Turbid liquid, ultrasonic, centrifugal separation, freeze-drying and other operations. The present invention uses natural tussah silkworm cocoons with excellent biocompatibility as raw materials to ensure the biosafety performance of nanocarriers, and the whole preparation method is simple and quick, and tussah silk nano-particles containing RGD peptide natural targeting ligands are prepared to avoid It avoids the severe environment and the toxic and side effects caused by the residue of organic solvents in the modification process of foreign ligands, and at the same time has a good function of targeting macrophages, the particle size distribution is relatively uniform, and it has high drug loading and encapsulation. It has a good application potential in the treatment of ulcerative colitis.

Description

technical field [0001] The invention relates to the technical field of nano drug carriers, in particular to a preparation method and application of tussah silk fibroin nanoparticles. Background technique [0002] Tussah silk fibroin (Antheraea pernyi silk fibroin, ApSF) protein is a natural polymer, which is only composed of H chains, according to the gene sequence of tussah silk fibroin (Sezutsu H, Yukuhiro K. Dynamic arrangement within the Antheraea pernyi silk The fibroin gene is associated with four types of repetitive units. J.Mol.Bio.2000; 51(4):329-338.) The amino acid sequence of the tussah silk fibroin protein deduced is composed of 2639 amino acid residues with a size of about 216kD composition. Alanine, glycine and serine are the three main amino acids constituting tussah silk fibroin, accounting for 43.09%, 27.26% and 11.25% of the total amino acids respectively. The head of tussah silk fibroin is the H chain, which consists of a residue sequence of 155 amino a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/42A61K31/05A61P1/04A61P1/00A61P29/00
CPCA61K9/5169A61K31/05A61P1/00A61P1/04A61P29/00
Inventor 肖波马雅
Owner SOUTHWEST UNIVERSITY
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