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Application of tangeretin in preparing oxidative stress protection product

An oxidative stress, citrus peeling technology, applied in the application, the function of food ingredients, medical preparations containing active ingredients, etc., to achieve the effect of increasing the ability to resist hydrogen peroxide stress damage

Pending Publication Date: 2019-10-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the improvement of tangeretin and the ability of cells to resist oxidative stress damage.

Method used

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  • Application of tangeretin in preparing oxidative stress protection product
  • Application of tangeretin in preparing oxidative stress protection product
  • Application of tangeretin in preparing oxidative stress protection product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Effect of Tangeretin on Cell Viability of Human Liver L02 Cells, Human Stomach GES Cells, Human Umbilical Vein Epithelial HUVEC Cells, and Human Fibroblast WI-38 Cells Injured by Hydrogen Peroxide Oxidative Stress

[0021] Human liver L02 cells, human gastric GES cells, human umbilical vein epithelial HUVEC cells, and human fibroblast WI-38 cells were cultured with RPMI-1640 containing 10% fetal bovine serum at 37°C and 5% CO 2 Incubate in a cell culture incubator, replace with fresh culture medium every other day, and passage once every 2-3 days. During the experiment, L02, GES, HUVEC, and WI-38 cells were seeded in 96-well plates. When the cells grow to 70-80% confluence, the original medium is discarded, and the drugs are added in groups. Set blank control group (without hydrogen peroxide treatment), DMSO solvent control group, N-acetylcysteine ​​(NAC) control group (final concentration is 2mmol / L) and tangeretin treatment group (final concentration is 0.5...

Embodiment 2

[0023] Example 2 Effects of Tangeretin on Intracellular Active Oxygen Content in Human Liver L02 Cells, Human Stomach GES Cells, Human Umbilical Vein Epithelial HUVEC Cells, and Human Fibroblast WI-38 Cells Injured by Hydrogen Peroxide Oxidative Stress

[0024] Human liver L02 cells, human gastric GES cells, human umbilical vein epithelial HUVEC cells, and human fibroblast WI-38 cells were cultured with RPMI-1640 containing 10% fetal bovine serum at 37°C and 5% CO 2Incubate in a cell culture incubator, replace with fresh culture medium every other day, and passage once every 2-3 days. During the experiment, L02, GES, HUVEC, and WI-38 cells were seeded in 96-well plates. When the cells grow to 70-80% confluence, the original medium is discarded, and the drugs are added in groups. Set up a blank control group (without hydrogen peroxide treatment), a DMSO solvent control group and different concentrations of tangeretin treatment groups (final concentrations were 0.5 μmol / L, 1 μm...

Embodiment 3

[0026] Example 3 Effect of Tangeretin on the Intracellular Antioxidative Enzyme Activity of Human Liver L02 Cells, Human Stomach GES Cells, Human Umbilical Vein Epithelial HUVEC Cells, and Human Fibroblast WI-38 Cells

[0027] Human liver L02 cells, human gastric GES cells, human umbilical vein epithelial HUVEC cells, and human fibroblast WI-38 cells were cultured with RPMI-1640 containing 10% fetal bovine serum at 37°C and 5% CO 2 Incubate in a cell culture incubator, replace with fresh culture medium every other day, and passage once every 2-3 days. During the experiment, L02, GES, HUVEC, and WI-38 cells were seeded in 96-well plates. When the cells grow to 70-80% confluence, the original medium is discarded, and the drugs are added in groups. Set up DMSO solvent control group and different concentrations of tangeretin treatment groups (final concentrations were 0.5 μmol / L, 1 μmol / L, 2 μmol / L, respectively). After acting for 6 hours, the culture medium containing tangereti...

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PUM

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Abstract

The invention provides an application of tangeretin in preparing an oxidative stress protection product. The ability of tangeretin to withstand hydrogen peroxide oxidative stress damage in human liverL02 cells, human gastric GES cells, human umbilical vein endothelial cells (HUVEC), and human fibroblast WI-38 cells is significantly increased, and the cells maintain the ability to withstand the oxidative stress damage after the tangeretin is removed; and the tangeretin can be used as a functional food, a health care product or a preventive drug for the prevention and treatment of diseases related to oxidative stress damage.

Description

technical field [0001] The invention belongs to the field of food and medicine, and relates to the application of tangeretin in the preparation of oxidative stress protection products. Background technique [0002] Oxidation and reduction are the most common chemical reactions in nature, manifested as electron gain or loss or shift of covalent electron pairs. Oxidative stress damage occurs when the accumulation rate of oxides in the organism exceeds the reduction reaction and the removal rate of oxides in the organism. Studies have shown that oxidative stress injury is associated with the occurrence and development of various diseases, including cancer, diabetes, obesity, nervous system diseases and atherosclerosis. [0003] Natural products can help the body to protect the body from oxidative stress damage, most of which have their own reducing effect, and are expressed as "bound" antioxidant natural active substances that react with active oxygen, which can enhance the ce...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P39/06A23L33/10
CPCA23L33/10A23V2002/00A23V2200/30A23V2250/21162A61K31/352A61P39/06
Inventor 孙崇德王岳曹锦萍李鲜陈昆松
Owner ZHEJIANG UNIV
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