A Class of Small Proteins and Their Applications

A protein and G protein technology, applied in the field of small proteins, can solve the problems of poor structural stability of polypeptide inhibitors, unfavorable for the design of small molecule inhibitors, no deep binding pockets, etc., to achieve weak IgG binding ability, no T cells. Depletion side effects, good tissue penetration effect
CN110305200BActive Publication Date: 2021-02-26SHANGHAI UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANGHAI UNIV
Publication Date
2021-02-26

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Abstract

The invention discloses a class of small proteins and applications thereof. The backbone protein of the small protein is a mutant of the IgG-binding domain of Streptococcal protein G, and the small protein includes an alpha-helix and four beta-sheets, the alpha-helix running through a region surrounded by four beta-sheets , and at the same time, key amino acid sites capable of binding to PD-1 protein are also distributed on the β-sheet surface formed by the four β-sheets. The small protein of the present invention has a high structural similarity with PD‑L1 protein, can inhibit PD‑1 / PD‑L1 binding, and is a potential PD‑1 / PD‑L1 inhibitor, which has weak IgG binding ability and can be used in vivo The circulation residence time is long, and has high structural stability, can be used to inhibit the interaction between PD‑1 / PD‑L1, is simple to prepare, can be used for large-scale production and application, and facilitates the immunotherapy of cancer.
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Description

technical field

[0001] The present invention relates to a class of small proteins, in particular to a class of small proteins based on the skeleton of GB1 (Immunoglobulin G-binding protein G, referred to as GB1 for short), and such small proteins At the same time, it has the ability to bind IgG, and its application as a PD-1 / PD-L1 inhibitor belongs to the technical field of PD-1 / PD-L1 inhibitors. Background technique

[0002] The immune system plays a vital role in the fight against cancer. Tumor cells express tumor-specific antigens on the cell surface due to changes in genetic material. Immune cells can kill tumor cells through the recognition of these specific antigens. However, tumor cells will adopt immune escape mechanisms to allow them to survive anti-tumor immunity. Therefore, blocking the escape of tumor cells is beneficial to the killing of tumor cells.

[0003] PD-1 (programmed cell death 1, CD279) programmed cell death protein is a type I membrane protein and...

Claims

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