Micro-nano compression device

Abstract

The invention relates to the technical field of biological science, particularly to a micro-nano compression device comprising an optical microscope, a glass substrate, a metal foil, a filling layer,a micro compressor, a liquid inlet tube, a liquid inlet, a liquid outlet tube, a liquid outlet, a protective layer, an electromagnet I, an electromagnet II, a voltage source and a cable. A small polymer sphere, a macromolecular sample, a small magnetic sphere and liquid are employed as compression experimental materials. The micro compressor includes a metal probe I, a metal probe II, a microchannel I, a microchannel II, a compression channel, a port I, a port II, a port III, and a port IV. The optical microscope is arranged at the position at a distance of 10 cm below the glass substrate. Themicrofluidic structure is combined with the magnetic force to apply a compression force on a biological target in confined space; the sample position is limited relatively during the compression process; and the compression effect is good. Moreover, the pressure application environment is similar to the original living environment of the biological sample; and the existing commercial optical microscope can be used for monitoring the compression process, so that sample deterioration is avoided.

Description

technical field [0001] The invention relates to the technical field of biological sciences, in particular to a micro-nano compression device for applying compression force to a biological target in a limited space and observing its response. Background technique [0002] In recent years, it has become more and more important to study the response of biological cells to external pressure stimuli at the molecular scale. Generally, the existing technology uses contact probe technology such as micro-press plate, micro-nano indentation technology, atomic force microscope, etc. The biological sample on the substrate exerts a unidirectional force, which has the disadvantage of causing the sample to move in the uncompressed direction and affecting the compression effect. Other existing technologies use optical traps to manipulate small balls located in microfluidic channels to The sample exerts a compressive force, which uses a laser to generate the force. However, since the local h...

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Problems solved by technology

[0002] In recent years, it has become more and more important to study the response of biological cells to external pressure stimuli at the molecular scale. Generally, the existing technology uses contact probe technology such as micro-press plate, micro-nano indentation technology, atomic force microscope, etc. The biological sample on the substrate exerts a unidirectional force, which has the disadvantage of causing the sample to move in the uncompressed direction, which affects the compression effect. Other existing technologies use optical traps to manipulate small balls located in microfluidic channels. The sample exerts a compressive force, which uses a laser to generate force. However, since the local heat generated by the laser will increase the temperature of the sample and destroy the original living environment of the biological sample, it is impossible to use a higher power laser, resulting in a The compression force is limited, the micro-nano compression device can solve the problem

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