Cell washing container, and method and system for cell collecting and washing

Abstract

The invention discloses a cell washing container, and a method and system for cell collecting and washing. The method includes pretreatment. A filter membrane is disposed in the cell washing containerand divides the inner cavity of the container into an upper cavity and a lower cavity. Negative pressure devices form negative pressure in the upper cavity to allow a cell suspension to be washed tobe in the upper cavity; liquid in the upper cavity is subjected to suction filtration to enter the lower cavity; normal saline is added into the upper cavity to resuspend cells; the abovementioned steps are repeated by a plurality of times until liquid meets a standard; a pre-cold cell preservation fluid is quantificationally added into the upper cavity and the cells are resuspended to complete cell washing. The system for cell collecting and washing includes the cell washing container, a normal saline container, a cell suspension container, an air filter, the first negative pressure device and the second negative pressure device. Beneficial effects are that: a manner combining negative pressure suction and the filter membrane is adopted to replace the cell suspension to finish cell enrichment and washing, the washing speed is high, and the efficiency of enrichment and washing is higher than that of traditional centrifugation and natural sedimentation manners and cell damage is lower.

Description

technical field

[0001] The invention relates to the technical field of cell culture, in particular to a cell washing container, a cell collection washing method and a system. Background technique

[0002] With the advancement of science and technology, cell therapy is gradually being developed clinically, which also promotes the development of cell culture and process processing technology. After large-scale culture, cells must be enriched, and the culture medium, nutrient solution and other components must be washed away, while maintaining their activity. The existing cell washing methods usually adopt centrifugation and natural sedimentation. Although the centrifugal method has high efficiency, it causes great damage to the cells. Although the natural sedimentation does little damage to the cells, the washing efficiency is very low. Therefore, how to efficiently enrich the cells Collecting and cleaning and minimizing cell damage are problems that need to be solved urgentl...

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