A kind of natural polysaccharide and freckle whitening application

A technology of natural polysaccharides and crude polysaccharides, which is applied in the field of natural polysaccharides and freckle-removing and whitening applications, and can solve the problems such as no report on the freckle-removing and whitening activity of polysaccharides from the rhizomes of cold flowers

Active Publication Date: 2020-07-17
广东诺品健康产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the freckle-removing and whitening activity of polysaccharides from the rhizome of Binglianghua

Method used

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  • A kind of natural polysaccharide and freckle whitening application
  • A kind of natural polysaccharide and freckle whitening application
  • A kind of natural polysaccharide and freckle whitening application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the preparation method of polysaccharide

[0028] 1. Experimental method

[0029] 1. Preparation of crude polysaccharide

[0030] Weigh 1 kg of dry ice-cold flower rhizomes (collected from Suihua City, Heilongjiang Province, my country, air-dry in a cool place after washing, and cut into sections), and first use 95% ethanol (volume percentage concentration, the same below) to degrease 3 times under heat reflux. 2 hours each time, then leached 3 times with 8L deionized water at 85°C, 2 hours each time, combined the water extracts, filtered, concentrated to 5L, and filtered again after standing for 24 hours, collected the supernatant, and added 5 times the volume of 95 % ethanol, stir evenly, let stand for alcohol precipitation for 24h, centrifuge (3000r / min×10min), collect the precipitate, redissolve with 3L deionized water, repeat the alcohol precipitation once, collect the precipitate, remove protein by Sevag method, and dialyze for 48h (dialysis The molec...

Embodiment 2

[0044] Example 2: Anti-freckle and whitening activity test

[0045] Human A-375 melanocytes are often used to evaluate the effect of external intervention factors on human freckle removal and whitening. The observation indicators include the inhibitory effect on the proliferation of human A-375 melanocytes and the inhibition of melanin synthesis in human A-375 melanocytes.

[0046] 1. Experimental materials

[0047] Polysaccharides BLH-1-JZ, BLH-2-JZ, and BLH-3-JZ were prepared according to the method in Example 1, and stored in a dry and cool place.

[0048] Human A-375 melanocytes were treated with DMEM / F12 medium (complete medium) containing 10% fetal bovine serum in 5% CO 2 , Culture at 37°C, when the cells grow to a confluence of about 85%, trypsinize and passage.

[0049] 2. Experimental method

[0050] 1. Determination of the inhibitory effect of polysaccharides on the proliferation of human A-375 melanocytes

[0051] Human A-375 melanocytes in the logarithmic growt...

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Abstract

The invention discloses natural polysaccharides and an application in preparation of freckle-removing whitening cosmetics. Melanin is a high-molecular biological pigment mainly distributed in human epidermal cells and keratinocytes and determines a skin color and a hair color of people, however, when the melanin is accumulated too much, problems such as color spots, freckles and dark skin can be caused. The invention provides three polysaccharides including BLH-1-JZ, BLH-2-JZ and BLH-3-JZ separated from rhizomes of adonis amurensis, the three polysaccharides have high purity, experiments findthat the three polysaccharides have excellent freckle-removing whitening activity (freckles-removing whitening mechanism is as follows: inhibition of proliferation of melanocytes and inhibition of melanin synthesis in the melanocytes), and the natural polysaccharides can be used to prepare the cosmetics such as freckle-removing whitening cream, freckle-removing whitening facial cleansers and freckle-removing whitening facial masks.

Description

technical field [0001] The invention relates to a natural polysaccharide and its application in removing freckles and whitening. Background technique [0002] Melanin is a high-molecular biopigment mainly distributed in human epidermal cells and keratinocytes, which determines human skin color and hair color (Zhang Huiying et al., Effects of grape seed proanthocyanidins on inhibiting melanin production in mouse B16-F0 melanoma cells, Chinese Medicine Pharmacology and Clinical Practice, 2017), but when melanin accumulates too much, it will not only cause problems such as spots and freckles, but also may become cancerous and produce melanoma. Therefore, the research and development of drugs and cosmetics that can be used to prevent and treat diseases such as pigmentation and melanoma have attracted the attention of researchers (Li Lili et al., New Progress in the Research of Tyrosinase Inhibitors, Food Industry, 2016). [0003] Polysaccharides are a large class of natural pol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K8/73A61Q19/02
CPCA61K8/73A61Q19/02C08B37/00C08B37/0003
Inventor 不公告发明人
Owner 广东诺品健康产业有限公司
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