Immobilized glutamine transaminase preparation method
A technology of glutamine and transaminase, applied in biochemical equipment and methods, acyltransferase, transferase and other directions, can solve problems such as easy loss of enzyme activity, and achieve the effects of low price, improved stability and reduced production cost
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[0037] Example 1
[0038] 1) Weigh a certain amount of transglutaminase proenzyme, add an appropriate amount of pure water to dissolve it to prepare an enzyme solution with an enzyme activity of 600 U / mL, the measured pH is 6.26, and the water activity is 0.97, sterilized for use.
[0039] 2) The natural polymer compound such as dextran gel is prepared into a suspension, which is heated to melt and sterilize.
[0040] 3) After cooling the sterilized carrier suspension to room temperature, mix it with 100 U / mL free glutamyl transaminase in a certain proportion, and let it stand in a refrigerator at 4° C. for 30 min.
[0041] 4) Wash twice with deionized water, add 1% glutaraldehyde solution to strengthen immobilization and crosslinking for 1 hour, remove glutaraldehyde, and wash twice with deionized water.
[0042] 5) Add the corresponding formula to the immobilized system according to Table 2 below to prepare immobilized transglutaminase.
[0043] Table 2 Formula of immobilized transglut...
Example Embodiment
[0046] Example 2
[0047] 1) Weigh a certain amount of transglutaminase proenzyme, add an appropriate amount of pure water to dissolve it to prepare an enzyme solution with an enzyme activity of 600 U / mL, the measured pH is 6.26, and the water activity is 0.97, sterilized for use.
[0048] 2) Prepare natural polymer compounds such as agarose gel into a suspension, and heat it to melt and sterilize.
[0049] 3) After cooling the sterilized carrier suspension to room temperature, mix it with 100 U / mL free glutamyl transaminase in a certain proportion, and let it stand in a refrigerator at 4° C. for 30 min.
[0050] 4) Wash twice with deionized water, add 1% glutaraldehyde solution to strengthen immobilization and crosslinking for 1 hour, remove glutaraldehyde, and wash twice with deionized water.
[0051] 5) Add the corresponding formula to the immobilized system according to Table 3 to prepare immobilized transglutaminase.
[0052] Table 3 Formula of immobilized transglutaminase
[0053] ...
Example Embodiment
[0055] Example 3
[0056] 1) Weigh a certain amount of transglutaminase proenzyme, add an appropriate amount of pure water to dissolve it to prepare an enzyme solution with an enzyme activity of 600 U / mL, the measured pH is 6.26, the water activity is 0.97, and it is sterilized for use.
[0057] 2) The natural polymer compound such as agarose gel is prepared into a suspension, which is heated to melt and sterilize.
[0058] 3) After cooling the sterilized carrier suspension to room temperature, mix it with 100 U / mL free glutamyl transaminase in a certain proportion, and let it stand in a refrigerator at 4° C. for 30 min.
[0059] 4) Wash twice with deionized water, add 1% glutaraldehyde solution to strengthen immobilization and crosslinking for 1 hour, remove glutaraldehyde, and wash twice with deionized water.
[0060] 5) Add the corresponding formula to the immobilized system according to Table 4 to prepare immobilized transglutaminase.
[0061] Table 4 Formula of immobilized transglut...
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