Primer-probe composition, reagent, kit and detection method for detecting human CYP2C 19 genotypes
A CYP2C19, primer-probe technology, used in biochemical equipment and methods, recombinant DNA technology, and microbial assay/inspection, etc., can solve the problems of false positives, expensive preparation, and expensive detection instruments, and achieve rapid specificity. sexual effect
Inactive Publication Date: 2019-10-29
WUXI SHENRUI BIO PHARMA
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The gene chip method to detect gene polymorphism is not only expensive to prepare and the cost of the detection instruments it depends on is even more expensive, but it also takes at least 8 hours to test CYP2C19 genotyping using the gene chip, and it is prone to false positives. Defect, that is, negative samples are misjudged as positive samples
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[0063] The present invention will be described in detail below in conjunction with the implementations shown in the drawings, but it should be noted that these implementations are not limitations of the present invention, and those of ordinary skill in the art based on the functions, methods, or structural changes made by these implementations Equivalent transformations or substitutions all fall within the protection scope of the present invention.
[0064] In this application, the term "sec" is a time unit: second; the term "min" is a time unit: minute.
[0065] The high-efficiency and specific detection for detecting the genotyping of human CYP2C19 gene loci due to population gene mutations disclosed in this application is used to determine the drugs involved in metabolism based on CYP2C19 (for example, proton pump inhibitors, anti-epileptic drugs, anti-epileptic drugs, etc.) Depressants, thienopyridines, antiplatelets, etc.) in different groups of people, and improve the dr...
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The invention provides a primer-probe composition, a reagent, a kit and a detection method for detecting human CYP2C19 genotypes, and belongs to the technical field of gene detection. In the primer-probe composition disclosed by the invention, a first primer-probe premixed liquid contains primers and probes with nucleotide sequences shown in SEQ ID NO. 1-4, a second primer-probe premixed liquid contains primers and probes with nucleotide sequences shown in SEQ ID NO. 5-8, and a third primer-probe premixed liquid contains primers and probes with nucleotide sequences shown in SEQ ID NO. 9-12. The primer-probe composition disclosed by the invention can effectively distinguish three genotypes, including CYP2C19 * 2, CYP2C19 * 3 and CYP2C19 * 17, through fluorescent quantitative PCR amplification, has the advantages of high specificity, high sensitivity, simple and quick operation, etc., and can shorten the detection time and reduce the detection cost for detecting the CYP2C19 genotypes, thereby providing an accurate basis for personalized medicine and improving the accuracy of genotype detection.
Description
technical field [0001] The invention relates to the technical field of gene detection, in particular to a primer probe composition, reagent, kit and detection method for detecting human CYP2C19 genotyping. Background technique [0002] CYP2C19 is an important member of the cytochrome P450 (CytochromeP450, CYP) enzyme system. P450 isoenzymes, also known as drug enzymes, are an enzyme family composed of a series of structurally and functionally related enzymes, and are the main enzyme system for drug metabolism in vivo. Studies have found that CYP2C19 can affect the metabolism of many important clinically used drugs such as clopidogrel, omeprazole, diazepam, and phenytoin, and its genetic polymorphisms cause different metabolism of the same drug between individuals and races. One of the reasons for the ability. In 2010, the US FDA required that the relationship between CYP2C19 and curative effect be indicated on the drug label of clopidogrel, and it was recommended to detect...
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IPC IPC(8): C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q2531/113C12Q2545/101C12Q2563/107C12Q2545/113
Inventor 盛青松姚鲁帅
Owner WUXI SHENRUI BIO PHARMA