Preparation method and application of umami peptide
A technology of umami peptides and reaction time, applied in the field of protein processing, can solve the problem of little enhancement of umami intensity
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Embodiment 1
[0035] (1) Extrusion: Send 20 kg of soybean protein isolate into a twin-screw extruder, set the temperature in zones 2 to 5 to 60°C, 80°C, 130°C and 150°C respectively, and set the temperature in zone 6 to 190°C. The feeding speed is 20kg·h -1 , the screw speed is 200r min-1, and the amount of water fed is 25%, to obtain denatured protein, which is dried and milled to pass through a 100-mesh sieve;
[0036] (2) Protease enzymatic hydrolysis: add deionized water to make a protein solution with a concentration of 70 mg / mL, adjust the pH to 9 with 1 mol / L NaOH, and then add 1000 U·g of enzyme specific substrate -1 Alkaline protease was reacted at 50°C for 1 hour. After the reaction, inactivate the enzyme in a 95°C water bath for 15 minutes, and quickly cooled to room temperature;
[0037] (3) Glutamic acid carboxypeptidase enzymatic hydrolysis: then add enzyme ratio substrate 2000U·g -1 The glutamic acid carboxypeptidase was reacted at 40°C for 1 h, and finally the enzyme was com...
Embodiment 2
[0040] (1) Extrusion: Send 20 kg of soybean protein isolate into a twin-screw extruder, set the temperatures in zones 2 to 5 to 60°C, 80°C, 130°C and 150°C respectively, and set the temperature in zone 6 to 200°C. The feeding speed is 25kg·h -1 , the screw speed is 220r min-1, the amount of water fed is 27%, the denatured protein is obtained, and it is dried and milled to pass through a 100-mesh sieve;
[0041] (2) Protease enzymatic hydrolysis: add deionized water to make a protein solution with a concentration of 70 mg / mL, adjust the pH to 9 with 1 mol / L NaOH, and then add 2000 U·g of enzyme substrate -1 Alkaline protease was reacted at 40°C for 2 hours. After the reaction, inactivate the enzyme in a 95°C water bath for 15 minutes, and quickly cooled to room temperature;
[0042] (3) Glutamic acid carboxypeptidase enzymatic hydrolysis: then add enzyme ratio substrate 2000U·g -1 The glutamic acid carboxypeptidase was reacted at 40°C for 1 h, and finally the enzyme was compl...
Embodiment 3
[0045] (1) Extrusion: Send 20 kg of soybean protein isolate into the twin-screw extruder, set the temperature in the second to fifth zones to 60°C, 80°C, 130°C and 150°C respectively, and set the temperature in the sixth zone to 210°C, The feeding speed is 30kg·h-1, the screw speed is 250r·min-1, the water feeding rate is 30%, the denatured protein is obtained, and it is dried and ground to pass through a 100-mesh sieve;
[0046] (2) Protease enzymatic hydrolysis: add deionized water to make a protein solution with a concentration of 70 mg / mL, adjust the pH to 9 with 1 mol / L NaOH, and then add 2000 U·g of enzyme substrate -1 Alkaline protease was reacted at 40°C for 2 hours. After the reaction, inactivate the enzyme in a 95°C water bath for 15 minutes, and quickly cooled to room temperature;
[0047] (3) Glutamic acid carboxypeptidase enzymatic hydrolysis: then add enzyme ratio substrate 2000U·g -1 The glutamic acid carboxypeptidase was reacted at 40°C for 2 hours, and finall...
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