An optimized fermentation process for production of hyaluronidase by Lactobacillus plantarum

Abstract

The invention relates to the technical field of microbial fermentation, in particular to a fermentation optimization process for producing hyaluronidase produced by Lactobacillus plantarum. The process comprises the following steps: preparing a seed medium; inserting Lactobacillus plantarum into the seed medium, and culturing to obtain liquid fermentation bacteria species; preparing a liquid fermentation medium; inserting the liquid fermentation strain into the liquid fermentation medium, and finishing the fermentation after culturing; centrifuging the fermentation liquid, and collecting the supernatant, which is the crude enzyme liquid. The present invention creatively adjusts the rotational speed and air flow of fermentation culture to carry out oxygen-resistant acclimation and cultivation on the bacterium, so that Lactobacillus plantarum can normally ferment and produce hyaluronidase under aerobic conditions; The yield of hyaluronidase in the product is greatly improved: the hyaluronidase contained in the fermentation broth obtained by this production process is 9604IU / mL, which is 40% higher than that before the process optimization, which is convenient for subsequent separation and purification; the production process of the present invention is simple and convenient. High stability, easy to scale up production and popularization.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a hyaluronidase fermentation optimization process produced by Lactobacillus plantarum. Background technique [0002] Hyaluronic acid is the main component of the extracellular matrix of the host's connective tissue, and hyaluronidase (HAase) can specifically decompose the extracellular matrix component - hyaluronic acid (HA) to produce low molecular weight A proteolytic enzyme. Hyaluronidase affects the proliferation, differentiation and migration of cells by acting on the extracellular matrix, and plays a role in the development of embryonic development and tumor development. Therefore, it plays a pivotal role in effectively controlling pathogen infection and preventing its spread. The prevention and treatment of bacterial infectious diseases is also of great significance. [0003] Although hyaluronidase is widely distributed, it has not been well developed and ...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem that there are few strains fermentable to produce hyaluronidase in the prior art and the enzyme activity is low, the present invention provides a fermentation optimization process for the production of hyaluronidase by Lactobacillus plantarum, which improves the composition of the medium and the culture conditions , increase hyaluronidase production and increase enzyme activity by 40%

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