Method for in vitro culture of Taishan Polygonatum sibiricum ovules

A culturing method and ovule technology are applied in the field of culturing ovules of Polygonatum taishani in vitro, which can solve the problems of non-germination and low harvest rate of mature seeds, and achieve the effects of reducing operation procedures, improving seedling reproduction efficiency and shortening reproduction process.

Inactive Publication Date: 2019-11-12
TAISHAN RES INST OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] Aiming at the above disadvantages, the present invention provides a method for cultivating isolated ovules of Polygonatum in vitro. This method obtains sterile seedlings faster than c

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0035] Implementation Mode 1: Direct Stripping

[0036] The vigorous Taishan Polygonatum field seedlings were selected as the test plants, and the young seeds free from diseases and insect pests at the 3-6 round leaves of the plant from the ground up 14-20 days after fertilization were used as explant materials. Young seeds are green and spherical, preferably 0.3-0.5 cm in length and width.

[0037]Firstly, the residual corolla and sepals coated on the surface of the young seeds are peeled off, rinsed with running water, and then aseptically sterilized.

[0038] First disinfect with 70% alcohol for 15-30s, then disinfect with 0.1% mercuric chloride solution for 7-10min, rinse with sterile water for 3-5 times, and then blot the surface dry for later use.

[0039] The aseptic ovules were obtained by direct stripping. The specific treatment method for the sterilized juvenile seed explants is as follows: starting from the abdominal suture of the ovary, cut the ovary wall to expo...

Embodiment approach 2

[0046] Embodiment 2: Transecting the ovary wall

[0047] The vigorously growing Taishan Polygonatum field seedlings were selected as the test plants, and the young seeds free from diseases and insect pests at the 3-6 wheel-shaped leaves of the plant from the ground up 14-20 days after fertilization were used as explant materials. The juvenile seeds are green and spherical, preferably 0.3-0.5 cm in length and width.

[0048] Firstly, the residual corolla and sepals coated on the surface of the young seeds are peeled off, rinsed with running water, and then aseptically sterilized.

[0049] First disinfect with 70% alcohol for 15-30s, then disinfect with 0.1% mercuric chloride solution for 7-10min, rinse with sterile water for 3-5 times, and then blot the surface dry for later use.

[0050] Aseptic ovules were obtained by transecting the ovary wall. The specific treatment method for the sterilized juvenile seed explants is as follows: cut off about 1 / 3-1 / 2 of the ovary wall by ...

Embodiment approach 3

[0054] Embodiment 3: Only part of the epidermis of the ovary wall is removed but the complete ovary is preserved

[0055] The vigorously growing Taishan Polygonatum field seedlings were selected as the test plants, and the young seeds free from diseases and insect pests at the 3-6 wheel-shaped leaves of the plant from the ground up 14-20 days after fertilization were used as explant materials. The juvenile seeds are green and spherical, preferably 0.3-0.5 cm in length and width. The culture conditions at this stage are as follows: first culture in the dark for 7-14 days, then transfer to normal light culture for 12 hours / day, and the temperature is about 25°C;

[0056] Firstly, the residual corolla and sepals coated on the surface of the young seeds are peeled off, rinsed with running water, and then aseptically sterilized. First disinfect with 70% alcohol for 15-30s, then disinfect with 0.1% mercuric chloride solution for 7-10min, rinse with sterile water for 3-5 times, and ...

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Abstract

The invention discloses a method for in vitro culture of Taishan Polygonatum sibiricum ovules. The method includes the following specific steps: taking young seeds of Taishan Polygonatum sibiricum field strong seedlings as explants, acquiring ovules by three treatment methods, and carrying out induced differentiation culture on the in vitro ovules to obtain regenerated plants, wherein the propagation coefficient can reach 7 at the highest. Compared with conventional sowing and propagation, the method can obtain sterile seedlings more quickly, and effectively solves the problems of low harvestrate of mature seeds in production, no germination during sowing in the current year, and the like. Breeding and seedling raising time is shortened, the materials are easily-available, disinfection iseasy, the method is of great significance for improving breeding and solving problems such as hybrid sterility, and further fill a gap in the research of a Taishan Polygonatum breeding technology.

Description

technical field [0001] The invention relates to the technical field of Taishan Polygonatum breeding, in particular to a method for cultivating isolated Taishan Polygonatum ovules. Background technique [0002] Taishan Polygonatum ( Polygonatum sibiricum ) is Asparagaceae (Asparagaceae) (originally belonged to Liliaceae (Liliaceae)) Polygonatum ( Polygonatum ) perennial herb with medicinal value, and Rhizoma Polygonatum ( Polygonatum kingianum ) and Polygonatum ( Polygonatum cyrtonema ) was also included as a medicinal variety by "Chinese Pharmacopoeia 2015". Indiscriminate mining and mining have been destroyed, and it has been difficult to meet market needs. Taishan Polygonatum can be propagated by seeds and rhizomes. At present, rhizomes are mainly used in production, and the reproductive efficiency is low, and it is easy to cause germplasm degradation and accumulation of diseases and insect pests. However, the use of seeds for propagation is limited by the extremely...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 黄艳艳王玉山仉劲
Owner TAISHAN RES INST OF FORESTRY
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