Calibration method of capacity of pipettor

Abstract

The invention discloses a calibration method of the capacity of a pipettor. According to the method, the capacity of the pipettor is calibrated by using ultraviolet visible spectrophotometry, the capacity of 1[mu] L or greater is calibrated in a single dyeing mode, and the capacity of the pipettor at a point to be calibrated is calculated according to a standard working curve and the absorbancy ofa solution at a specific wavelength; and in a double-dyeing mode, the capacity of 1[mu] L or less is calibrated, a micropore shade guide is adopted for measurement, the volume of a diluent in the double-dye mode is obtained by measuring the absorbancy of the solution at 730nm through conversion, the uncertainty introduced in the addition process of the diluent is eliminated, and the measurement accuracy is improved. Compared with the prior art, on one hand, the calibration method overcomes the influence of natural evaporation of a liquid in a measuring method, and requirements on environmentconditions are reduced; and on the other hand, in the single dyeing mode and the double-dyeing mode, accurate calibration of capacities of 1[mu] L or greater and 1[mu] L or lower can be simultaneouslyachieved, and calibration requirements of different accuracy grades can be met.

Description

technical field [0001] The invention relates to the technical field of pipettes, in particular to a method for calibrating the capacity of pipettes. Background technique [0002] As one of the commonly used micro-liquid volume measuring instruments, pipettes are widely used in hospitals, health and epidemic prevention stations, blood transfusion stations, biochemical laboratories, environmental laboratories, and food analysis laboratories due to their portability and simple operation. , which is used more and more widely. It belongs to the precision liquid sampling instrument, which can quickly and accurately quantitatively sample and add samples to a small amount of liquid samples and test solutions. For adjustable pipettes, the operator can also adjust the pipette according to actual needs. The capacity value of the device. Especially with the rapid development of biopharmaceuticals, genetic engineering and molecular diagnosis, the industry has higher and higher requireme...

AI Technical Summary

Problems solved by technology

This method needs to be equipped with a precision electronic balance with a division value of 0.001 mg, and has high requirements on the use environment, such as temperature, pressure, humidity, etc. Generally, it can only be measured in a precision constant temperature laboratory, and during the measurement process, calibration The smaller the capacity, the greater the impact caused by the natural evaporation of the liquid, and manual operation will also bring a certain error

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