Multi-nozzle synergistic bioprinting method

Abstract

The multi-nozzle collaborative bioprinting method performs the following operations: calibrate the nozzles of all nozzle components to the origin of the world coordinate system, determine the nozzle components that need to perform printing tasks, and the nozzle components start printing tasks from the origin in turn; or, the nozzle components perform printing tasks sequentially , the end point of the task path of the previous nozzle assembly is the starting point of the task path of the next nozzle assembly; or, all the nozzle assemblies performing the printing task execute the same printing path, and after all the nozzle assemblies reach the zero position, they will start from the zero position along the printing path synchronously. Start the print job. The advantages of the present invention are: the system has multiple nozzle assemblies that can work together or sequentially, and the printing mode is flexible and changeable; the nozzle tip of the nozzle assembly is cleaned and cleaned, and the stable output of materials in the formal printing task is guaranteed; The surrounding temperature control of the vessel and the vacuum adsorption clamping at the bottom of the vessel can effectively guarantee the quality of the formed living structure and improve the survival rate of the living tissue.

Description

technical field [0001] The invention relates to the technical field of biological 3D printing in tissue engineering, in particular to a multi-nozzle collaborative biological printing method of a high-precision biological printing device. Background technique [0002] Every year in the world, an extremely large number of people suffer from various types of injuries leading to tissue defects, or some major diseases require organ transplantation, resulting in a huge demand for tissue and organ repair. For the treatment of damaged large soft tissues and internal organs in the human body, tissue and organ transplantation is an extremely effective treatment method. However, due to the shortage of organ donor sources, immune rejection and other problems, there are insurmountable difficulties in the practical application of organ transplantation. The proposal of tissue engineering has opened up a new way to solve the above problems. Tissue engineering is to attach living cells to ...

AI Technical Summary

Problems solved by technology

For another example, when printing skin, it is necessary to print blood vessels and other tissues. If a single nozzle is used, it will take a long time to replace the nozzle, which will affect the efficiency, and it is also possible that the hydrogel on the other side may be damaged just after the nozzle is replaced. If it is solidified, if multiple infusion channels of the same nozzle are used, it will cause the problem of material mixing at the nozzle; and it cannot be printed at the same time, and the efficiency is lower

[0005] The single nozzle on the market takes envisionTec as an example. Every time the material is changed, the nozzle needs to be moved to the new nozzle, and then the tool is changed by vacuum adsorption, which has the problems of long time and unguaranteed positioning accuracy.

In addition, if there are gaps between the 6 blocks when printing a discrete hexagonal shape similar to liver cells, there will be longer wiring; this severely limits the ability to print complex organs, and the printing time is too long and the efficiency is low

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