Probiotics-containing breast milk-like formula milk powder for infants and preparation method of milk powder
An infant formula and probiotic technology, applied in dairy products, milk preparations, bacteria used in food preparation, etc., can solve the problem of low proportion of families, and achieve the goal of promoting brain development, improving memory and intelligence, and maintaining intestinal cells. The effect of tight connection
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Embodiment 1-8
[0087] Embodiment 1-8 A kind of breastmilkized infant formula milk powder containing probiotics
[0088] Examples 1-8 are respectively a kind of breastmilkized infant formula milk powder containing probiotics, and the raw materials for making them are shown in Table 1 below:
[0089] Table 1
[0090]
[0091] In embodiment 1-8, the content of Bifidobacterium lactis HN019, Bifidobacterium animalis Bb-12 and Lactobacillus paracasei N1115 is 1×10 11 ~1×10 12 CFU / g. The above-mentioned Lactobacillus paracasei N1115 was isolated and screened from traditional fermented milk products in Inner Mongolia, China, and was preserved in the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences in March 2011 with the preservation number CGMCC4691.
[0092] In Examples 1-8, the multivitamin contains the following components: vitamin A, vitamin D, vitamin E, vitamin K 1 , Vitamin B 2 , Vitamin B 6 , Vitamin B 12 , niacinamide, folic acid, vitamin C,...
Embodiment 9-14
[0093] Example 9-14 A preparation method of breastmilkized infant formula milk powder containing probiotics
[0094] Embodiment 9-14 is used to prepare any embodiment of embodiment 1-embodiment 8, and the preparation process is carried out according to the following steps:
[0095] 1. Raw milk acceptance and clean milk
[0096] Weigh raw milk according to the weight in Examples 1-8 respectively, standardize the raw milk to obtain material A, and then separate impurities from material A to obtain material B;
[0097] 2. Pre-killing
[0098] Put B at temperature T 1 Carry out pasteurization under the temperature, then cool down, obtain material C;
[0099] 3. Wet mixing
[0100] According to the weight in Examples 1-8, correspondingly weigh desalted whey powder, white granulated sugar, anhydrous butter, soybean oil, lactose, concentrated whey protein powder, galacto-oligosaccharide, fructo-oligosaccharide, 3' sialic acid Lactose, phospholipids, calcium carbonate, nucleotide...
Embodiment 15
[0115] Example 15 Application of Lactobacillus paracasei N1115 to promote intestinal development
[0116] 1. Evaluation of intestinal barrier function by Lactobacillus paracasei N1115
[0117] Intestinal development is extremely important in the growth of infants and young children, providing essential nutrients for young children. In addition to the proliferation and differentiation of intestinal cells, some probiotics in the intestinal tract can maintain intestinal cell activity by regulating intestinal permeability, thereby To achieve the purpose of maintaining the tight junction structure between intestinal cells, based on this, this example adopts the Caco-2 cell research model in vitro, and cultures Caco-2 on the upper layer of the Transwell filter membrane. With the prolongation of the culture time, the Caco-2 cells gradually Contact each other, differentiate, and finally form a tight junction structure between the cells, and the measured transmembrane resistance value ...
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