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Milk fat globule membrane rich in phospholipid, preparation method of milk fat globule membrane and application of milk fat globule membrane in promoting brain development and relieving cognitive function decline

A technology of milk fat globule film and milk fat, which is applied in the field of food science, can solve the problems of lack, poor thermal stability, and inability to simulate human milk fat digestion and absorption well

Pending Publication Date: 2021-10-01
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Fat in infant formula milk powder is the main component that provides energy for infants and young children. In order to reduce fatty acid oxidation and improve its stability, there have been reports that cyclodextrin and whey protein have been used as wall materials in infant formula milk powder. However, the particle size of fat globules obtained after high-pressure homogeneous emulsification is significantly smaller than that of human milk fat globules, which cannot well simulate the digestion and absorption of human milk fat
In addition to the difference in the size of the fat globules, the surface of the fat globules obtained by this method lacks active components such as phospholipids and glycoproteins in the milk fat globule membrane, and has poor thermal stability.

Method used

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  • Milk fat globule membrane rich in phospholipid, preparation method of milk fat globule membrane and application of milk fat globule membrane in promoting brain development and relieving cognitive function decline
  • Milk fat globule membrane rich in phospholipid, preparation method of milk fat globule membrane and application of milk fat globule membrane in promoting brain development and relieving cognitive function decline
  • Milk fat globule membrane rich in phospholipid, preparation method of milk fat globule membrane and application of milk fat globule membrane in promoting brain development and relieving cognitive function decline

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1, the preparation of the milk fat globule membrane powder that is rich in fat globule membrane phospholipid

[0067] 1. 2L of fresh raw Holstein milk taken within 24 hours of milk collection;

[0068] 2. According to the temperature of the raw milk, heat or cool it in a water bath to 20°C, then pour it into the ultrasonic treatment device, and put water at 25°C into the side chamber to reduce the heating rate of the milk during processing;

[0069] 3. Treat the raw milk with a vertical plate transducer at a frequency of 1MHz and a power of 189W / L for 30 minutes to obtain the milk fat extract of the upper layer;

[0070] 4. Store the milk fat extract obtained in step 3 at 4° C. for 20 hours, stir with a stirrer immediately after cooling and crystallization, the stirrer speed is 600 rpm, and the stirring time is 30 min;

[0071] 5. Pass the stirred product obtained in step 4 through a filter screen with a pore size of 0.71 μm to obtain a milk fat globule memb...

Embodiment 2

[0074] Embodiment 2, the preparation of the milk fat globule film powder that is rich in phospholipid

[0075] 1. 4L of fresh raw yak milk taken within 24 hours of milk collection;

[0076] 2. According to the temperature of the raw milk, heat or cool it in a water bath to 23°C, then pour it into the ultrasonic treatment device, and put water at 25°C into the side chamber to reduce the heating rate of the milk during processing;

[0077] 3. Treat the raw milk with a vertical plate transducer at a frequency of 2MHz and a power of 157W / L for 20 minutes to obtain the milk fat extract of the upper layer;

[0078] 4. Store the milk fat extract obtained in step 3 at 4° C. for 20 hours, stir with a stirrer immediately after cooling and crystallization, the stirrer rotating speed is 700 rpm, and the stirring time is 20 min;

[0079] 5. Pass the stirred product obtained in step 4 through a filter screen with a pore size of 0.71 μm to obtain a milk fat globule membrane extract;

[008...

Embodiment 3

[0082] Embodiment 3, the preparation of the milk fat globule film powder that is rich in phospholipid

[0083] 1. 6L of fresh raw goat milk taken within 24 hours of milk collection;

[0084] Two, filter with 4 layers of gauze, collect the filtrate, use a centrifuge to centrifuge at room temperature, the speed and time of centrifugation are respectively 3000rpm / min, 20min, obtain fat crude extract;

[0085] Three, add a certain amount of PBS phosphate buffer saline in the fat crude extract, add the amount of PBS phosphate buffer saline by fat extract: PBS phosphate buffer saline volume ratio is 1:9 (v / v) adds, PBS phosphate buffer solution formula is 0.018M Na 2 HPO 4 and NaH 2 PO 4 , 0.12M NaCl, adjusted to pH 7.0. After fully mixing, use a centrifuge to perform centrifugal washing to wash off impurities on the surface of fat globules and separate to obtain fat extracts. This process was repeated 3 times.

[0086] 4. Store the milk fat extract obtained in step 3 at 4° C...

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Abstract

The invention discloses a milk fat globule membrane rich in phospholipid, a preparation method of the milk fat globule membrane and application of the milk fat globule membrane in promoting brain development, improving learning ability, relieving cognitive function decline and preventing Alzheimer's disease. The method comprises the following steps: separating from fresh raw milk to obtain a milk fat extract; cooling and crystallizing, stirring, and passing through a filter screen with the aperture of 0.65-0.80 mu m to obtain a milk fat globule membrane extract; adding ethanol into the milk fat globule membrane extract for ultrasonication and centrifugation I; taking supernate, removing the solvent, adding cold acetone, centrifuging II to remove non-polar lipid, and removing residual cold acetone, thereby obtaining the product. The milk fat globule membrane rich in phospholipid has a good application prospect in preparation of functional foods or health care products such as infant formula milk powder and pregnant and lying-in woman milk powder with the effects of promoting brain development and improving cognitive functions, and middle-aged and elderly formula milk powder for preventing cognitive function decline and relieving Alzheimer's disease.

Description

technical field [0001] The invention relates to a phospholipid-rich milk fat globule membrane, a preparation method thereof and an application thereof in promoting brain development and alleviating cognitive function decline, and belongs to the field of food science and technology. Background technique [0002] Milk is a natural oil-in-water emulsion, and its oil phase mainly contains fat globules with a diameter of about 0.1-10 μm. These fat globules are protected from enzymatic degradation and aggregation by Milk Fat Globule Membrane (MFGM). The natural fat globule membrane in raw milk is mainly composed of protein and lipid. Almost all the polar lipids in milk come from the fat globule membrane, which accounts for about 33% of the total fat globule membrane lipids. It has a variety of biological functions, can improve the digestion and absorption rate of fat, and has anticancer and anti-inflammatory activities. , Reduce the ability to absorb cholesterol, improve brain d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C21/06A23C21/08A23C21/10B01J19/10C11B1/00
CPCA23C21/06A23C21/08A23C21/10C11B1/00B01J19/10
Inventor 毛学英袁绮晨巩涵李天歌赵丽丽刘泽朋
Owner CHINA AGRI UNIV
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