Method for biological three-dimensional printing of intervertebral disc in liquid support medium

A technology of supporting medium and three-dimensional printing, applied in the field of three-dimensional printing, can solve the problems of short duration of curative effect and inability to cure, and achieve the effect of improving stability and high precision

Pending Publication Date: 2019-11-29
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the treatment of lumbar disc herniation is divided into two categories: non-surgical treatment and surgical treatment. Non-surgical treatment mainly includes drug treatment, traction treatment, physical therapy, closed treatment, etc. These methods mainly relieve symptoms, and the duration of curative effect is relatively short. Can't be cured

Method used

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  • Method for biological three-dimensional printing of intervertebral disc in liquid support medium
  • Method for biological three-dimensional printing of intervertebral disc in liquid support medium
  • Method for biological three-dimensional printing of intervertebral disc in liquid support medium

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Experimental program
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Effect test

Embodiment 1

[0033] The method for biological three-dimensional printing of an intervertebral disc in a liquid support medium comprises the following steps:

[0034] Step S101, cultivation of seed cells: culture in F12 medium containing 10% fetal bovine serum at 37°C, 5% CO2 incubator, with 10% fetal bovine serum, connective tissue growth factor CTGF25ng / ml, ascorbic acid 25uM The α-MEM medium was cultured in a 37°C, 5% CO2 incubator.

[0035] Step S103, preparation of supporting solvent: Measure appropriate amount of chitosan, alginic acid, cyclodextrin, starch, albumin and gelatin, dissolve in solvent and adjust to desired density.

[0036] Step S105, establishment of the artificial intervertebral disc printing model: use the 3D printing software to establish the printing model, the outer layer is a multi-layer ring, the inner layer is a multi-layer spiral, and the endplate cartilage is a multi-layer rectangle, save it and wait for the next step.

[0037] Step S107, preparation of artif...

Embodiment 2

[0042]Biomacromolecule materials A1, A2, A3, and A4 are made of one of polylactic acid, polycaprolactone, polylactic acid-glycolic acid copolymer, polylactic acid-caprolactone copolymer, polydioxanone or Several compositions, further, natural materials B1, B2, B3, and B4 are composed of one or more of gelatin, hyaluronic acid, sodium alginate, chitosan, and collagen, and the seed cells are composed of bone marrow stem cells, One or more components of chondrocytes, the cross-linking agent is one or more of ultraviolet light and calcium chloride solution, after the artificial intervertebral disc is printed, a solution containing alginate lyase is used to remove alginate Support medium.

Embodiment 3

[0044] (1) Use 3D printing software to build a printing model. The outer layer is in the shape of a multi-layer ring, and the inner layer is in a multi-layer spiral shape. The path gaps in the nucleus pulposus area and the annulus fibrosus area are different. Save it and wait for the next step;

[0045] (2) Take an appropriate amount of alginate, xanthan gum, and calcium carbonate, dissolve in D-(+) glucono δ lactone and double distilled water, and adjust to the required density;

[0046] (3) Measure an appropriate amount of polycaprolactone and sterilize it. Measure an appropriate amount of gelatin and sterilize it, mix it with bone marrow stem cells evenly, and obtain a gelatin gel containing bone marrow stem cells, and wait for the next step;

[0047] (4) Equipment preparation: adjust the process parameters of the biological three-dimensional printing equipment: the diameter of the print head is 100 μm, the printing temperature of polycaprolactone is 120 ° C, the printing t...

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Abstract

The invention discloses a method for biological three-dimensional printing of intervertebral disc in a liquid support medium. The liquid support medium for the biological three-dimensional printing ofintervertebral disc is prepared from the following raw materials in parts by mass: 5-50 parts of chitosan, 5-50 parts of alginic acid, 5-50 parts of cyclodextrin, 5-50 parts of starch, 5-50 parts ofalbumin, 5-50 parts of gelatin, and 5-50 parts of a solvent. The method has the advantages that: the method can accurately control the printing path and greatly improve the stability of the printing structure; and uses a printing liquid containing seed cells to obtain the intervertebral disc biological scaffold containing a large number of cells, designs printing path parameters according to requirements, and obtains the artificial bionic intervertebral disc biological scaffold by printing. The intervertebral disc biological scaffold obtained by the method has an endplate cartilage-annulus fibrosus-nucleus pulposus structure, has high structure precision, carries a large amount of seed cells for proliferation and repair, and is beneficial to rapidly establishing a microenvironment around an intervertebral disc and reconstructing the intervertebral disc structure and function.

Description

technical field [0001] The invention relates to the technical field of three-dimensional printing, in particular to a method for biological three-dimensional printing of an intervertebral disc in a liquid support medium. Background technique [0002] The lumbar intervertebral disc is composed of three parts: the nucleus pulposus, the fibrous annulus and the cartilage plate. It is located between the upper and lower vertebrae and plays a role in supporting the rotation and reducing the coefficient of friction during the lumbar movement. Lumbar disc herniation is a relatively common motor system injury, and its incidence is increasing year by year. The cause is mainly that after a certain degree of degenerative changes in the lumbar intervertebral disc, the annulus fibrosus ruptures under the external force, and the nucleus pulposus tissue compresses the tissue in the spinal canal, causing the adjacent spinal nerve root to be stimulated or compressed, resulting in waist pain, ...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): A61L27/26A61L27/38B29C64/106B29C64/393B33Y50/02B33Y70/00
CPCA61L27/26A61L27/3834A61L27/3817B29C64/106B29C64/393B33Y50/02B33Y70/00A61L2300/412
Inventor乔之光唐佳昕韩煜连梅菲孙彬彬戴尅戎孙晓江赵杰
OwnerSHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE