A kind of blood group antibody complex, preparation method

A blood group antibody and complex technology, which is applied in the field of extracting a blood group antibody complex and its preparation, can solve the problems of difficulty in obtaining anti-AB antibodies, large randomness, time-consuming and labor-intensive, etc., and achieves simple operation, low cost and good biological activity. Effect

Active Publication Date: 2022-07-19
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, anti-AB antibodies are difficult to obtain. Generally, through lymphocyte hybridoma technology, anti-AB antibodies that react with type A, B, and AB red blood cells, and do not...

Method used

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  • A kind of blood group antibody complex, preparation method
  • A kind of blood group antibody complex, preparation method
  • A kind of blood group antibody complex, preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Prepare a blood group antibody complex with the structure of mouse anti-A blood group monoclonal antibody-goat anti-mouse polyclonal antibody-mouse anti-B blood group monoclonal antibody, such as figure 1 shown, the steps are as follows:

[0035] S1. Select mouse anti-A blood group monoclonal antibody and mouse anti-B blood group monoclonal antibody as blood group antibody I and blood group antibody II respectively;

[0036] S2. Select the goat anti-mouse polyclonal antibody that can specifically bind to the mouse anti-A blood group monoclonal antibody and the mouse anti-B blood group monoclonal antibody at the same time as the bridged secondary antibody;

[0037] S3. Mix the mouse anti-A blood group monoclonal antibody, the mouse anti-B blood group monoclonal antibody, and the goat anti-mouse polyclonal antibody 1:1:1 according to the number of molar equivalents, and place it at room temperature for 15-30 minutes to obtain the figure 2 The structure shown is a blood ...

Embodiment 2

[0040] Prepare a blood group antibody complex with the structure of mouse anti-B-1 blood group monoclonal antibody-goat anti-mouse polyclonal antibody-mouse anti-B-2 blood group monoclonal antibody, such as figure 1 shown, the steps are as follows:

[0041] S1. Select the same titer of mouse anti-B-1 blood group monoclonal antibody and mouse anti-B-2 blood group monoclonal antibody secreted by two different cell lines as blood group antibody I and blood group antibody II, respectively;

[0042] S2. Select the goat anti-mouse polyclonal antibody that can specifically bind to the mouse anti-B-1 blood group monoclonal antibody and the mouse anti-B-2 blood group monoclonal antibody at the same time as the bridged secondary antibody;

[0043] S3. Mix the mouse anti-B-1 blood group monoclonal antibody, the mouse anti-B-2 blood group monoclonal antibody, and the goat anti-mouse polyclonal antibody in a 1:1:1 molar equivalent, and place it at room temperature for 15-30 minutes to obta...

Embodiment 3

[0054] The method for preparing the blood group antibody complex with the structure of mouse anti-A-1 blood group monoclonal antibody-goat anti-mouse polyclonal antibody-mouse anti-A-2 blood group monoclonal antibody is the same as that in Example 2. The same titers of mouse anti-A-1 blood group monoclonal antibody and mouse anti-A-2 blood group monoclonal antibody secreted by two different cell lines were selected as blood group antibody I and blood group antibody II, respectively. The prepared structure is that the blood group antibody complex of mouse anti-A-1 blood group monoclonal antibody-goat anti-mouse polyclonal antibody-mouse anti-A-2 blood group monoclonal antibody has higher titer than anti-A-1 monoclonal antibody or anti-A-2 monoclonal antibody.

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Abstract

The present invention provides a blood group antibody complex whose structure is blood group antibody I-bridged secondary antibody-blood group antibody II. The present invention also provides a method for preparing the above-mentioned blood group antibody complex, comprising the following steps: S1, selecting two antibodies derived from the same biological species, namely blood group antibody I and blood group antibody II; S2, according to the blood group antibody Species sources of I and blood group antibody II, select a bridged secondary antibody that can specifically bind to blood group I and blood group antibody II at the same time; Mix evenly at a ratio of 1:1:1, and place at room temperature for 15-30 minutes to prepare a complex with the structure of blood group antibody I-bridged secondary antibody-blood group antibody II. The preparation method of the blood group antibody complex provided by the present invention can increase the specificity of the blood group antibody or improve the affinity and titer of the blood group antibody by utilizing the specific recognition between the antibody and its specific antibody, and the operation is simple and fast. Wide range of applications.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a blood group antibody complex and a preparation method. Background technique [0002] The immune response ability of antibodies includes three aspects: antibody titer, antibody affinity, and antibody specificity. "Antibody titer" is a biological term. It is a reaction that uses the physical state of the antibody and its residence time in the body to indicate its immune effect by how much it reacts with the antigen. In layman's terms, it means that the antibody can be detected. Maximum dilution factor. "Antibody affinity" refers to the degree of binding between an antibody molecule and the entire antigen. "Antibody specificity" refers to the selective recognition of a specific epitope of an antigen by an antibody. [0003] For blood group antibodies to be used in clinical blood group detection, their titer and affinity are very important indicators. To improve the titer and affin...

Claims

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Application Information

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IPC IPC(8): G01N33/80G01N33/68
CPCG01N33/80G01N33/6845
Inventor 陈晔洲李勇王红梅段生宝丁少华魏双施
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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