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Liquid chromatography tandem mass spectrometry method for quantitatively detecting catecholamine metabolite

A technology of catecholamines and metabolites, which is applied in the field of liquid chromatography tandem mass spectrometry, can solve the problems of excessively prolonging the detection time and inhibiting application, and achieve the effects of avoiding missed diagnosis, high sensitivity and specificity

Inactive Publication Date: 2019-12-13
重庆黄嘉同怡科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the existing technology uses a combination of different liquid chromatography tandem mass spectrometry methods to detect such substances, this leads to an excessively prolonged detection time, which largely inhibits its clinical application

Method used

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  • Liquid chromatography tandem mass spectrometry method for quantitatively detecting catecholamine metabolite
  • Liquid chromatography tandem mass spectrometry method for quantitatively detecting catecholamine metabolite
  • Liquid chromatography tandem mass spectrometry method for quantitatively detecting catecholamine metabolite

Examples

Experimental program
Comparison scheme
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Embodiment

[0022] Mix 500 μL ammonium phosphate solution (25 mmol / L), 25 μL internal standard solution of catecholamine metabolites (MN-d3, NMN-d3, 3-MT-d4, concentration 3 ng / mL), 500 μL biological sample, vortex well After mixing, the sample solution to be tested was formed for use; 500 μL standard solution of catecholamine metabolites and their metabolites (MN, NMN, 3-MT) with 5 gradient concentrations were mixed with 500 μL ammonium phosphate solution (25 mmol / L), 25 μL The internal standard solutions of catecholamine metabolites (MN-d3, NMN-d3, 3-MT-d4, the concentration is 3ng / mL) were mixed evenly to form 5 standard mixed solutions respectively; the metabolites of catecholamine metabolites (MN , NMN, 3-MT) 5 gradient concentrations were: 50, 100, 200, 500, 800pg / mL, the weak cation exchange 96-well solid phase extraction plate was pretreated with methanol and ammonium phosphate solution respectively, and then respectively Add the above mixed sample solution and standard solution i...

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Abstract

The invention discloses a liquid chromatography tandem mass spectrometry method for quantitatively detecting catecholamine metabolite. The method can be used to rapidly and effectively detect the contents of metanephrine, normetanephrine and 3-methoxytyramine in a biological sample. The first-initiated index in the association guide can be detected, and effective detection and auxiliary diagnosiseffects can be achieved. The detection range is expanded, and the detection method can be used to diagnose various types of PPGLs and can avoid missed diagnosis. In addition, the detection method hasvery high sensitivity and specificity when used in diagnosis, and the sensitivity and the specificity can reach 98.7% and 97.3% respectively.

Description

technical field [0001] The invention relates to the technology in the field of trace compound detection, in particular to a liquid chromatography tandem mass spectrometry method for quantitative detection of catecholamine metabolites. Background technique [0002] Pheochromocytoma and paraganglioma (PPGL) are neuroendocrine tumors that arise from sympathetic or parasympathetic ganglion cells in the adrenal medulla and extra-adrenal gland, respectively. Tumors usually secrete a large amount of catecholamines paroxysmally or persistently, causing paroxysmal or persistent elevation of blood pressure in patients. The clinical manifestations of pheochromocytoma and paraganglioma are extremely heterogeneous. Although patients account for only 0.05%-0.2% of adult hypertensive patients, if these patients are not detected in time and receive targeted treatment, long-term hypertension It will cause serious damage to the heart, brain, kidney and other organs of the patient. If the di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/36G01N30/86
CPCG01N30/02G01N30/06G01N30/36G01N30/8675
Inventor 李小勇
Owner 重庆黄嘉同怡科技有限公司