Cultivation method of oudemansiella raphanipes
A cultivation method and technology of black-skinned chicken, which are applied in cultivation, plant cultivation, mushroom cultivation and other directions, can solve the problems of scarce yield, low fruiting rate, and difficulty in effectively increasing yield and the like.
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Embodiment 1
[0023] A kind of method for cultivating P. nigella, comprising the following steps:
[0024] S1. Breeding of mother species:
[0025] a. Take 1000ml of nutrient solution, 22-25g of agar, 35-50ml of sweet potato extract and mix to make the mother seed medium; The associated water is mixed according to the ratio of 28:25:2:2:1:2:1000 and boiled on low heat for 2 hours, then filtered, and the filtrate is taken as the nutrient solution. % of dry distiller's grains, wherein the associated water is obtained by mixing wild Gallus firsiformis mud and water in a ratio of 1:50 and heating on medium heat for 30 minutes and then filtering; the preparation method of the sweet potato extract is as follows: cut fresh sweet potatoes into pieces After soaking in water for 2-5 hours, take it out and mash it into a slurry, then put it into a centrifuge for centrifugation and remove the supernatant liquid, then filter to get the filtrate, which is the sweet potato extract;
[0026] B. get a par...
Embodiment 2
[0036] The difference between this example and Example 1 is that the nutrient solution is composed of distiller's grains, sorghum husks, aloe, potassium dihydrogen phosphate, magnesium sulfate, peptone, and associated water according to the ratio of 28:25:4:2:1:2:1000 Proportionally mixed and simmered for 2 hours, then filtered, and the filtrate was taken as the nutrient solution.
Embodiment 3
[0039] In order to verify the influence of aloe breeding on the fruiting rate: a special alignment group is set for comparison:
[0040] Control group:
[0041] The difference between this control group and Example 1 is that the nutrient solution is mixed with distiller's grains, sorghum husks, potassium dihydrogen phosphate, magnesium sulfate, peptone, and associated water in a ratio of 28:25:2:1:2:1000 and mixed in small amounts. Filtrate after boiling for 2 hours, and take the filtrate as nutrient solution.
[0042] Extract 200 inoculated bacterium sticks respectively in embodiment one, embodiment two, embodiment three and the control group; in the same greenhouse, adopt the same cultivation scheme and cultivation conditions, and cultivate respectively in subregions; after cultivating half a year Harvest in a unified manner, and count the fruiting situation of their respective mushroom sticks as shown in Table 1.
[0043] Table 1: Fruiting situation table
[0044]
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