Establishment method of fingerprint of moringa oleifera extract and fingerprint obtained through establishment method

A technology of Moringa extract and fingerprints, which is applied in the field of traditional Chinese medicine analysis, can solve the problems of low content of active ingredients in extracts, low extraction efficiency of Moringa leaves, and low content of active ingredients in ingredients, and achieve rich fingerprint information and baseline Smooth, well-structured results

Active Publication Date: 2019-12-20
广州泽力医药科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] So far, there has been little research on the fingerprints of Moringa oleifera leaves and related products. The main reasons are: the extraction efficiency of Moringa oleifera leaves is low, the content of medicinal ingredients in the extract is low, and it is difficult to separate the components in the fingerprints , the basic research on its chemical composition is weak, which restricts the research on its quality control standards, and the content of active ingredients in the extracted components is small, which limits the research process of its quality standards

Method used

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  • Establishment method of fingerprint of moringa oleifera extract and fingerprint obtained through establishment method
  • Establishment method of fingerprint of moringa oleifera extract and fingerprint obtained through establishment method
  • Establishment method of fingerprint of moringa oleifera extract and fingerprint obtained through establishment method

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Experimental program
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Embodiment 1

[0042] The preparation of embodiment 1 Moringa oleifera extract

[0043] The preparation of Moringa oleifera extract at least comprises the following steps:

[0044] (1) raw material pre-treatment: take the Moringa medicinal material, use a pulverizer to pulverize it and pass through a mesh sieve, the aperture of the mesh sieve is 0.8-1.2mm, to obtain the Moringa oleifera powder;

[0045] (2) Extraction: Use high-efficiency high-pressure differential low-temperature continuous extraction equipment, add water according to the ratio of medicinal material to liquid mass ratio of 1:15-50 for extraction, the extraction temperature is ≤30°C, and the extraction pressure is ≥20Mpa

[0046] Obtain Moringa extract;

[0047] (3) Centrifugation: the Moringa oleifera extract is centrifuged in a centrifuge, and the centrifuge speed is 3500-15000r / min to obtain the Moringa oleifera centrifugate;

[0048] (4) Filtration: the Moringa centrifugate is microwaved with a ceramic membrane with an...

Embodiment 2

[0057] The method for establishing the fingerprint of the Moringa oleifera extract of embodiment 2

[0058] 1. Experimental equipment and reagents

[0059] Equipment: Agilengt1100 series high-performance liquid chromatography (including DAD array detector, Agilent Company of the United States), Diamonsil C18 chromatographic column (4.6mm×250mm, 5μm), DV215CD electronic balance (Ohaus Company of the United States)

[0060] Reagents: acetonitrile, methanol (chromatographically pure, Fisher, USA), phosphoric acid (analytical pure, Chengdu Kelong Chemical Reagent Factory), reference substance isoquercitrin (China Institute for Food and Drug Control, batch number 100080-200707), gallic acid (Chengdu Pusi Biotechnology Co., Ltd., batch number P80380030), super pure water.

[0061] Two, the preparation of test solution and reference solution

[0062] a. Preparation of the test solution: Moringa oleifera extract samples come from 12 batches of Moringa oleifera extract samples produc...

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Abstract

The invention provides an establishment method of a fingerprint of a moringa oleifera extract and the fingerprint obtained through the establishment method and relates to the technical field of traditional Chinese medicine analysis. According to the establishment method, a test solution is prepared from a moringa oleifera extract sample; a solution into which a gallic acid reference substance andan isoquercitrin reference substance are dissolved is taken as a reference substance solution; liquid chromatography is determined through a high performance liquid chromatograph; the obtained liquidchromatography is introduced into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system to be analyzed; and the fingerprint of the moringa oleifera extract is obtained through analysis. According to the establishment method of the fingerprint of the moringa oleifera extract provided by the invention, for the characteristics of the moringa oleifera extract, the established fingerprint is comprehensive in information, various peaks in the fingerprint are high in separation degree, 20 common peaks are calibrated, a base line is stable, the quality of the moringaoleifera extract can be objectively, comprehensively and accurately evaluated through a detection method, and the detection method is of great significance for control on the quality of the moringa oleifera extract and guarantee of the quality of a product.

Description

【Technical field】 [0001] The invention relates to the technical field of traditional Chinese medicine analysis, in particular to a method for establishing fingerprints of Moringa oleifera extract and the fingerprints thereof. 【Background technique】 [0002] Moringa, also known as the drumstick tree, is native to India and is known as the magical tree of "the tree of life", "the diamond in plants" and "the gold of vegetarian food" in the world. Indian traditional medicine believes that Moringa leaves have the effects of protecting the liver, diuresis, anti-inflammatory, pain relief, lowering blood pressure, strengthening the heart, and promoting lactation. It is often used to prevent and treat diabetes, hypertension, skin diseases, weak immunity, anemia, and scurvy , obesity, arthritis, digestive organ tumors and other diseases, it is a new resource food raw material rich in flavonoids and polyphenols, which can be used in food, health care products, cosmetics and other indus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/14G01N30/88
CPCG01N30/02G01N30/14G01N30/88G01N2030/146G01N2030/8813
Inventor 刘常青段海霞宋力飞刘乡乡唐晓纯
Owner 广州泽力医药科技有限公司
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