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Tobacco seedling culture medium and preparation method and application thereof

A seedling-raising substrate and tobacco technology, which is applied in the field of tobacco seedling-raising substrate and its preparation, can solve the problems of long cultivation period of tobacco seedlings and the reduction of tobacco seedling growth due to the porosity of the substrate, so as to reduce the pressure on peat resources, expand resource utilization methods, and promote The effect of premature hair growth

Inactive Publication Date: 2019-12-31
湖南省烟草公司永州市公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the salt content in the biogas residue is high and the particle size is small. Excessive use will easily cause the porosity of the substrate to decrease, affect the growth of tobacco seedlings, and even burn the seedlings.
[0004] In addition, the cultivation cycle of tobacco seedlings is long, generally takes about 80 days, and the cultivation period is from December to March, and the temperature is relatively low. Therefore, measures to promote the growth and early development of tobacco seedlings under low temperature conditions have become the best way to improve the seedling rate and quality of tobacco seedlings. The essential

Method used

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  • Tobacco seedling culture medium and preparation method and application thereof
  • Tobacco seedling culture medium and preparation method and application thereof
  • Tobacco seedling culture medium and preparation method and application thereof

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preparation example Construction

[0027] The invention provides the preparation method of the tobacco seedling raising substrate, comprising the following steps: 1) mixing biogas residue, peat, vermiculite and perlite to obtain a premixed substrate; 2) inoculating Pseudomonas WXX into the premixed substrate After -1, mix and stack to obtain the tobacco seedling cultivation substrate. In the present invention, there is no special limitation on the mixing method of the premixed matrix, and the conventional mixing method in the field can be used for mixing. In the present invention, the stacking time is preferably 4-6d, more preferably 5d; the stacking temperature is preferably 28-30°C. .

[0028] The invention also provides the application of the tobacco seedling raising substrate in promoting the growth of tobacco seedlings. In the present invention, the tobacco seedling-raising substrate can significantly increase the root length, stem length, maximum leaf length, maximum leaf width, fresh weight and dry wei...

Embodiment 1

[0031] Preparation of Pseudomonas protegens WXX-1 suspension

[0032] The preservation number of Pseudomonas protegens WXX-1 is CGMCCNo.11539.

[0033] ① Streak and activate the WXX-1 strain stored in the glycerol tube on the NB solid plate, and cultivate it in a 30°C incubator for 24 hours. ②Pick a single colony into a 4ml liquid NB test tube, culture at 30°C and 170rpm for 24 hours with shaking, and use the bacterial solution as the seed solution. ③Transfer the seed solution to the liquid NB shake flask with an inoculation amount of 1% (v / v), and cultivate it at 30°C and 180rpm until the mid-logarithmic phase (OD 600 >1.0), the cells were collected by centrifugation at 4°C, washed three times with distilled water, and resuspended in an equal volume of distilled water to obtain a bacterial suspension. ④ Preparation of NB liquid medium: beef extract 5g / L, peptone 10g / L, sodium chloride 5g / L. For NB solid medium, 20g / L of agar is added on the basis of liquid medium.

[0034...

Embodiment 2

[0047] Detection of the growth-promoting effect of the tobacco seedling raising substrate in Example 1 and Comparative Examples 1-4

[0048] The tobacco variety is Yunyan 87. Select plump and consistent tobacco seeds, culture them in a petri dish at 30°C for 2 days to accelerate germination, add vermiculite to the plugs and sow 3-5 grains of consistent white seeds (1cm deep), and wait until the tobacco grows 4 leaves 1 After the heart, select tobacco seedlings of the same size and transplant them to the seedling substrate.

[0049] The seedling cultivation test was carried out in a controllable incubator, simulating the actual tobacco greenhouse seedling cultivation, and the settings were divided into two stages: the first stage was 25°C, light for 16h, light intensity 18000Lux, humidity 75%; the second stage was 20°C, dark for 8h, Humidity 90%.

[0050] Tobacco seedlings were cultivated in a standard 50-hole tray (53cm×27cm), each hole was filled with 16g of tobacco seedling...

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Abstract

The invention belongs to the technical field of tobacco cultivation and provides a tobacco seedling culture medium and a preparation method and application thereof. The tobacco seedling culture mediumcomprises a premix matrix and Pseudomonas protegens WXX-1; a mass volume ratio of the premix matrix to the Pseudomonas protegens WXX-1 is (8-12)kg: 1L; the premix matrix is prepared from biogas residues, peat, vermiculite and perlite; a preservation number of the Pseudomonas protegens WXX-1 is CGMCC No.11539. The tobacco seedling culture medium contains rich nutrients, the Pseudomonas protegens WXX-1 is capable of secreting growth activity promoting substances, and early growth and quick tillering of tobacco seedlings can be promoted under low-temperature conditions. According to records in an embodiment, compared with seedlings cultured in a special commercial culture medium for tobaccos, the tobacco seedling culture medium has the advantage that fresh weight is increased by 155%.

Description

technical field [0001] The invention belongs to the technical field of tobacco cultivation, and in particular relates to a tobacco seedling cultivation substrate and a preparation method and application thereof. Background technique [0002] In the substrate for raising tobacco seedlings, peat is the main raw material, and generally the proportion in the substrate formula is 50% to 70%, which is more suitable. Since peat is a non-renewable resource with limited reserves, in order to reduce the pressure on peat resources, in the current research on seedling substrates, other materials are mostly used to replace peat to reduce the amount of peat added. Substitute materials include crop straw, bran, fly ash, and earthworms. Manure, etc., the proportion of peat can be appropriately reduced. However, straws need to be carbonized or processed with feces and peat to be safely used. The treatment process is not only cumbersome, but also increases labor costs. Therefore, it is neces...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G24/28A01G24/15A01G24/20A01G22/45
CPCA01G24/28A01G24/15A01G24/20A01G22/45
Inventor 王兴祥张静周志高靳志丽李孝刚丁昌峰
Owner 湖南省烟草公司永州市公司
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