Culture method for increasing in-vitro survival rate of mammal embryos

Abstract

The invention discloses a culture method for increasing the in vitro survival rate of mammal embryos. The method comprises the following steps: (1) taking sheep oocytes from ewes, putting the sheep oocytes into a maturation culture solution, and performing culturing in an incubator for 20-24 hours to obtain maturated sheep oocytes; (2) putting the mature sheep oocytes and sperms into a fertilization culture solution together, performing culturing in an incubator for 18-24 hours, and performing fertilizing to obtain early sheep embryos; and (3) repeatedly washing the fertilized sheep embryos with a TCM199 culture solution, then putting the sheep embryos into a sheep embryo in-vitro culture solution, putting the sheep embryos into an incubator for embryo in-vitro culture, and maintaining thetemperature in the incubator at 38-39 DEG C and the gas concentration at 5% CO2. The incubator comprises a culture chamber, a chamber door and a gas concentration adjusting chamber communicated withthe culture chamber; and a plurality of culture bins are arranged in the culture chamber, and the plurality of culture bins are respectively provided with a culture medium placing rack and a partitiondevice. The environment in the incubator is stable, so that the survival rate of the embryos is guaranteed.

Description

Technical field [0001] The invention belongs to the technical field of in vitro culture of sheep embryos, and particularly relates to a culture method for improving the in vitro survival rate of mammalian embryos. Background technique [0002] In vitro fetal culture refers to placing embryos in an incubator. The incubator maintains a constant temperature and a constant concentration of carbon dioxide to simulate the physiological environment of the uterus. The embryos in the culture medium interact with and exchange substances with them to achieve embryo growth. In the existing culture method, after the embryo is placed in the incubator, the operator needs to frequently observe or operate the embryo. At this time, the door of the incubator needs to be opened, and the environment inside the incubator will be changed during the operation. The gas exchange with the outside air causes the environment inside the box to be affected and change. It is difficult to ensure that the incubat...

AI Technical Summary

Problems solved by technology

In the existing culture method, after the embryos are placed in the incubator, the operator needs to observe or operate the embryos frequently. At this time, the door of the incubator needs to be opened, and during the operation, the environment in the box will change. The gas exchange with the outside air makes the environment in the box affected and changes, it is difficult to ensure that the incubator is always maintained in a stable temperature and atmosphere environment, the embryos are prone to damage or death, and the embryo survival rate is low

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