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Application of SUCLG2 (Succinate-CoA Ligase GDP-Forming Beta Subunit) autoantibody detection reagent in preparing lung cancer screening kit

An autoantibody and kit technology, applied in biological tests, measurement devices, material testing products, etc., can solve the problems of no SUCLG2 protein, no lung cancer, etc., and achieve good application prospects, effective screening, and low patient harm. Effect

Inactive Publication Date: 2019-12-31
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no related reports on SUCLG2 protein autoantibodies, and there is no prior art related to lung cancer

Method used

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  • Application of SUCLG2 (Succinate-CoA Ligase GDP-Forming Beta Subunit) autoantibody detection reagent in preparing lung cancer screening kit
  • Application of SUCLG2 (Succinate-CoA Ligase GDP-Forming Beta Subunit) autoantibody detection reagent in preparing lung cancer screening kit
  • Application of SUCLG2 (Succinate-CoA Ligase GDP-Forming Beta Subunit) autoantibody detection reagent in preparing lung cancer screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Relationship Between SUCLG2 Autoantibodies in Plasma and Lung Cancer

[0027] 1. Clinical data

[0028] 30 lung cancer patients and 29 healthy controls were selected. The basic information is as follows:

[0029] Basic Information lung cancer patients healthy control number of people 30 29 age 58±10.5 42±8.9 male ratio 20(66.7％) 13(46.7％)

[0030] 2. Detection principle

[0031] HuProt TM SUCLG2 protein is immobilized on the human protein custom chip (the SUCLG2 protein used is a full-length protein, and the protein sequence number is Ensembl: ENSP00000417589). After incubation with serum, the SUCLG2 autoantibodies (mainly including IgG and IgM antibodies, and some Other types of antibodies) will bind to it, wash to remove unbound antibodies and other proteins, and then detect with anti-human IgM fluorescently labeled secondary antibody (cy5-labeled, red) and anti-human IgG fluorescent secondary antibody (cy3-labeled, ...

Embodiment 2

[0047] Embodiment 2 The composition of detection kit of the present invention and its using method

[0048] 1. Kit composition

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Abstract

The invention relates to the field of in vitro diagnostic reagents, and in particular to an application of a SUCLG2 (Succinate-CoA Ligase GDP-Forming Beta Subunit) autoantibody detection reagent in preparing a lung cancer screening kit. According to the application of the SUCLG2 autoantibody detection reagent in preparing the lung cancer screening kit provided by the invention, the situation thatthe autoantibody level of SUCLG2 proteins in the serum of a lung cancer patient is significantly lower than that of a healthy control is found for the first time. The reagent for detecting the SUCLG2protein autoantibody is used for preparing the lung cancer screening kit, and effective screening of lung cancer can be implemented.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to the use of SUCLG2 autoantibody detection reagents in the preparation of lung cancer screening kits. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] Lung cancer is a disease that is good at concealment. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they are diagnosed with lung cancer symptoms. The best cure time, five-year survival rate is low. For patients with early lung cancer, timely treatment can greatly improve the 5-year survival rate and quality of life of the patients. Therefore, early diagnosis and effective screening of lung cance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/68G01N33/535
CPCG01N33/57423G01N33/57488G01N33/6854G01N33/6884G01N33/535G01N2333/4746
Inventor 李蕊岑黄燕刘芳露
Owner WEST CHINA HOSPITAL SICHUAN UNIV