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Dendrobium huoshanense capsule preservation method for seedling tissue culture

A preservation method, the technology of Dendrobium huoshanense, applied in the fields of plant preservation, botanical equipment and methods, and application, can solve the problems of idleness, unbalanced labor, missed spring cultivation opportunities, etc. The effect of the probability of carrying bacteria

Inactive Publication Date: 2020-01-07
安徽祥枫生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because the sowing time of Dendrobium Huoshanense is relatively concentrated, the cultivation cycle of Dendrobium Huoshanense group is 10-12 months; if it is sown in November, it will not be able to emerge until the autumn of the next year, which misses the best time for spring cultivation, and Due to the concentrated sowing time, the transfer of seedlings is very concentrated, resulting in a large number of skilled workers who need to invest in centralized transfer to complete the task, and after the transfer, a large number of them are idle, resulting in unbalanced employment

Method used

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  • Dendrobium huoshanense capsule preservation method for seedling tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for preserving dendrobium huoshanense capsules for group cultivation, comprising the following steps:

[0040] (1) At the end of October, before the capsules of Dendrobium Huoshanense were ripe and cracked, choose sunny weather to harvest the capsules of Dendrobium Huoshanense;

[0041] (2) Pretreatment of Dendrobium Huoshanense capsules: remove the necrotic tissue of Dendrobium Huoshanense capsules, sterilize by ultraviolet light under dry conditions for 1 hour, and turn over once every 10 minutes during the sterilization process;

[0042] (3) Processing of storage containers:

[0043] a. Wipe the refrigerator with 75% alcohol for disinfection, then open the door to dry, close the door and sterilize with ultraviolet light for 30 minutes, and seal it for later use;

[0044] b. Wipe the drying dish with 75% alcohol, and then place it on a clean operating table to dry;

[0045] c. Arrange the gauze at 121°C for 20 minutes and then dry the gauze at 80°C for 2 ho...

Embodiment 2

[0058] A method for preserving dendrobium huoshanense capsules for group cultivation, comprising the following steps:

[0059] (1) At the beginning of November, before the capsules of Dendrobium Huoshanense are ripe and cracked, choose sunny weather to harvest the capsules of Dendrobium Huoshanense;

[0060] (2) Pretreatment of Dendrobium Huoshanense capsules: remove the necrotic tissue of Dendrobium Huoshanense capsules, sterilize by ultraviolet light under dry conditions for 1 hour, and turn over once every 10 minutes during the sterilization process;

[0061] (3) Processing of storage containers:

[0062] a. Wipe the refrigerator with 75% alcohol for disinfection, then open the door to dry, close the door and sterilize with ultraviolet light for 30 minutes, and seal it for later use;

[0063] b. Wipe the drying dish with 75% alcohol, and then put it on a clean operating table to dry.

[0064] c. Arrange the gauze at 121°C for 20 minutes and then dry the gauze at 80°C for ...

Embodiment 3

[0077] A method for preserving dendrobium huoshanense capsules for group cultivation, comprising the following steps:

[0078] (1) At the beginning of November, before the capsules of Dendrobium Huoshanense are ripe and cracked, choose sunny weather to harvest the capsules of Dendrobium Huoshanense;

[0079] (2) Pretreatment of Dendrobium Huoshanense capsules: remove the necrotic tissue of Dendrobium Huoshanense capsules, sterilize by ultraviolet light under dry conditions for 1 hour, and turn over once every 10 minutes during the sterilization process;

[0080] (3) Processing of storage containers:

[0081] a. Wipe the refrigerator with 75% alcohol for disinfection, then open the door to dry, close the door and sterilize with ultraviolet light for 30 minutes, and seal it for later use;

[0082] b. Wipe the drying dish with 75% alcohol, and then put it on a clean operating table to dry.

[0083] c. Arrange the gauze at 121°C for 20 minutes and then dry the gauze at 80°C for ...

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Abstract

The invention discloses a Dendrobium huoshanense capsule preservation method for seedling tissue culture and relates to the technical field of Dendrobium huoshanense. The method includes the steps offirstly, picking Dendrobium huoshanense capsules; secondly, preprocessing the Dendrobium huoshanense capsules; thirdly, processing a preservation container which is a drying vessel; fourthly, on a sterile operation platform, wrapping the preprocessed Dendrobium huoshanense capsules obtained in the second step with a dry breathable material, placing a drying agent into the drying vessel, and placing the wrapped Dendrobium huoshanense capsules into the drying vessel; fifthly, preserving the drying vessel loaded with the Dendrobium huoshanense capsules into a refrigerated container. The method has the advantages that the cold-storage preservation method is adopted, excessive water loss, which is caused by dewatering and affects seed germination rate, of seeds can be prevented, and the preservation time of the Dendrobium huoshanense capsules can reach up to 12 months.

Description

technical field [0001] The invention relates to the technical field of Dendrobium Huoshanense, in particular to a method for preserving Dendrobium Huoshanense capsules for group cultivation. Background technique [0002] Dendrobium Huoshanense capsules usually mature from October to early November. The capsules are oval and contain tens of thousands to hundreds of thousands of seeds. When the capsules are fully mature, they will split from the pedicle of the tail and the seeds will be blown by the wind. out. Therefore, the best time to harvest the capsules of Dendrobium huoshanense is the early stage of full maturity. The best sowing time for group-cultivated seedlings is within one month after harvesting. Because the capsules of Dendrobium huoshanense are not easy to store after harvesting, they will be dehydrated and cracked, and the cracked capsules will not be disinfected thoroughly, and do not meet the requirements for aseptic sowing, so they cannot be used. Therefor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01C1/08A01N3/00
CPCA01C1/00A01C1/08A01N3/00
Inventor 高久双戴斯博兰柳梅王分红刘柳贵
Owner 安徽祥枫生物科技有限公司
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