Film strip for identifying mycobacterium tuberculosis, mycobacterium chelonae, mycobacterium abscessus, and preparation and use methods of film strip

A technology of Mycobacterium tuberculosis and Mycobacterium, applied in the direction of biochemical equipment and methods, microbiological measurement/inspection, etc., can solve the problems of inability to distinguish Mycobacterium chelonis and Mycobacterium abscessus, complicated operation steps, etc., and achieve The effect of good clinical application prospects

Pending Publication Date: 2020-01-07
TIANJIN HAIHE HOSPITAL
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, there are gene chip kits on the market for detecting mycobacterium strains, which adopt the principle of detecting 16SrRNA gene sequence, and the probes are fluorescently labeled, but they...

Method used

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  • Film strip for identifying mycobacterium tuberculosis, mycobacterium chelonae, mycobacterium abscessus, and preparation and use methods of film strip
  • Film strip for identifying mycobacterium tuberculosis, mycobacterium chelonae, mycobacterium abscessus, and preparation and use methods of film strip
  • Film strip for identifying mycobacterium tuberculosis, mycobacterium chelonae, mycobacterium abscessus, and preparation and use methods of film strip

Examples

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Effect test

Embodiment 1

[0041] Example 1. Establishment of detection method:

[0042] 1. Bacteria: The standard strain of Mycobacterium tuberculosis H37Rv preserved in the Mycobacterium strain bank of Tianjin Haihe Hospital, and the clinically isolated and cultured tortoise and Mycobacterium abscesses strains, among which Mycobacterium chelonae and Mycobacterium abscesses adopt Beijing Bo The gene chip method mycobacterial species identification kit produced by Ao Biological Group is obtained by using the ITS primer of the present invention to amplify and sequence after the identification.

[0043] 2. Extraction of bacterial DNA

[0044] 1ml of mycobacterial strain solution, centrifuged at 12000rpm for 5min, washed the precipitated part with physiological saline twice, discarded the supernatant and resuspended the precipitated part with 100μl of TE buffer, sonicated the sonicator at 300W for 15min, heated at 100℃ for 10min, 12000rpm After centrifugation for 5 min, the supernatant is the bacterial DNA solut...

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Abstract

The present invention belongs to the field of microbial gene detection and particularly relates to a film strip for identifying mycobacterium tuberculosis, mycobacterium chelonae, mycobacterium abscessus, and preparation and use methods of the film strip. Specific probes comprise base sequences for detecting the mycobacterium tuberculosis in SEQ ID NO.1-2; base sequences for detecting the mycobacterium chelonae in SEQ ID NO.3-4; base sequences for detecting the mycobacterium abscessus in SEQ ID NO.5-6 and mycobacterium quality control probes SEQ ID NO.7-8; and corresponding primer pairs are SEQ ID NO.9-10. The established detection method can rapidly and accurately distinguish the mycobacterium tuberculosis, mycobacterium chelonae and mycobacterium abscessus, can be used as a useful supplementary identification method for a commercialized mycobacterium strain identification kit, and has good clinical application prospects.

Description

Technical field [0001] The invention belongs to the field of microbial gene detection, and specifically relates to a film strip for identifying Mycobacterium tuberculosis, Mycobacterium chelonae, and Mycobacterium abscesses, and a preparation and use method. Background technique [0002] Tuberculosis caused by Mycobacterium tuberculosis (M.TB) infection is still one of the most common infectious diseases in my country. Non-tuberculous mycobacterial disease refers to other branches except Mycobacterium tuberculosis and Mycobacterium leprosy Bacillus infection causes lesions of related tissues and organs. Berger’s Manual of Systematic Bacteriology divides nontuberculous mycobacteria (NTM) into fast-growing and slow-growing types according to their growth rate. Among the diseases caused by fast-growing NTM in my country, the most common type of bacteria is turtle / abscess. Mycobacterium. At present, there are already marketed gene chip kits for detecting Mycobacterium strains, which ...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/6834C12Q1/04
CPCC12Q1/6834C12Q1/689C12Q2600/166C12Q2531/113C12Q2545/113
Inventor 郭明日谢祎武俊平张丽霞李妍
Owner TIANJIN HAIHE HOSPITAL
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