Chronic food-borne Parkinson's disease mouse model and building method and application thereof

Abstract

The invention belongs to the technical field of animal model building and discloses a chronic food-borne Parkinson's disease mouse model and a building method and application thereof. The method includes: a 5% ferric citrate solution is used to continuous intervene a C57BL / 6 mouse for 16 weeks, behavioral evaluation is performed through open-field, speed-up rotation and pole climbing experiments every other 4 weeks, and the mouse of the 16th week is subjected to a suspension experiment and a food award type Y-maze experiment; the measured mouse total journey, average speed, center times, center journey, center time and on-rod time of the mouse are evidently reduced as compared with those of the control, turning time and pole climbing time of the mouse are evidently increased as compared with those of the control, the results are time-dependent, and mouse sports coordination, balance ability, muscular tension and activity are lowered evidently. The chronic food-borne Parkinson's diseasemouse model and the building method have the advantages that the occurrence conditions of Parkinson's disease are simulated, and the chronic model is suitable for monitoring early-stage change and screening the early-stage diagnosis indexes of the Parkinson's disease; behavioral disorder can be quantified, and the pathological characteristic of the Parkinson's disease is dopaminergic neuron degeneration and loss characteristic lesion.

Description

Technical field [0001] The invention belongs to the technical field of animal model establishment, and in particular relates to a chronic food-borne Parkinson's disease mouse model and its establishment method and application. Background technique [0002] At present, the closest existing technology: The current PD models mainly include chemical drug models, biological toxic substance models, protease inhibitor models, gene models, common MPTP mouse models, rotenone models, 6- Hydroxydopamine (6-OHDA) model, intraperitoneal injection of iron dextran model, intracerebroventricular injection of FC or ferric chloride model, gene knockout and other PD models are basically acute or subacute models, and there are difficulties in making PD models and behaviors It cannot be quantified by science, is acute and reversible, has large side effects, some animals are not sensitive, and the success rate is low. Each model has its own advantages and disadvantages, and the most suitable model sh...

AI Technical Summary

Problems solved by technology

In the past few decades, toxin-based PD rodent models are basically acute or subacute models. Although they have been widely used to study the pathogenesis and pharmacodynamics of PD, they rarely include all of PD. Clinical behavior disorders and pathological features, and side effects, difficult operation, research results and evaluation results are not ideal, so it is necessary to establish a PD animal model with a complete phenotype

[0007] To sum up, the problems existing in the existing technology are: currently existing PD models are difficult to make PD models, acutely reversible, have large side effects, behavior cannot be quantified, some animals are not sensitive, and the success rate is low

In the past few decades, toxin-based PD rodent models are basically acute or subacute models. Although they have been widely used to study the pathogenesis and pharmacodynamics of PD, they rarely include all of PD. Clinical behavior disorders and pathological features, and side effects, difficult operation, research results and evaluation results are not ideal, so it is necessary to establish a PD animal model with a complete phenotype

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