Rhizospheric prebiotics composition for reducing incidence rate of tomato bacterial wilt and application of composition
A prebiotic and composition technology, applied in the field of rhizosphere prebiotic composition, can solve problems such as failure to achieve effects, and achieve the effects of reduced investment, easy availability of raw materials, and better comprehensive prevention and control effects.
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Embodiment 1
[0026] Embodiment 1. Obtaining of prebiotic formula
[0027] The rhizosphere soil samples of healthy and bacterial wilt diseased tomato were collected from the tomato experimental field of Nanjing Institute of Vegetables, and the rhizosphere metabolome of healthy and diseased tomato was determined by gas chromatography-mass spectrometry (GC-MS). The results showed that there were significant differences in metabolites in the rhizosphere of healthy and diseased tomatoes, and the substances significantly enriched in the rhizosphere of healthy tomatoes included melibiose, fructose, sucrose, ribose, xylose, mannose, maltose, gluconolactone, Inositol, Adonitol, Isoleucine, Alanine, Aspartic Acid, Tryptophan. Subdivision found that these 14 substances belong to 4 categories, namely sugars (melibiose, fructose, sucrose, ribose, xylose, mannose, maltose), esters (gluconolactone), alcohols (muscleose alcohol, adonitol), amides (isoleucine, alanine, aspartic acid, tryptophan). Therefo...
Embodiment 2
[0029] Example 2. Further optimization of prebiotic formula
[0030] With reference to the experimental method of Example 1, further studies have found that when the combination of prebiotics contains more than 7 (including 7) substances, its effect is not significantly different from that of all 10 substances, such as figure 2 Shown are the inhibitory effects of three preferred formulations comprising more than 7 substances (see Table 1 for formulation) and all 10 formulations of substances on R. solanacearum in soil.
[0031] Table 1 Three preferred prebiotic formulations
[0032] formula Substance type 1 Melibiose, fructose, ribose, xylose, mannose, maltose, inositol 2 Fructose, Sucrose, Ribose, Xylose, Mannose, Maltose, Adonitol 3 Melibiose, fructose, sucrose, ribose, xylose, mannose, maltose
Embodiment 3
[0033] Example 3. The application of rhizosphere prebiotics can help tomato resist the invasion of pathogenic bacteria
[0034] 3.1 Applying rhizosphere prebiotics to reduce the incidence of tomato bacterial wilt
[0035] The tomato seedlings that have been cultivated for about one month are transplanted into 32 hole hole trays (the depth of the hole in each hole is 5cm, the diameter is 6cm, and the bottom is 3cm), and 50g of soil containing Ralstonia solanacearum is added to each hole (Ralstonia solanacearum bacterium liquid The concentration is 5*10 7 CFU / g). One week after transplanting, an aqueous solution of prebiotics was added to the rhizosphere of each tomato hole, so that the final concentration of prebiotics was 50 μmol / kg, and the experimental control was the treatment of adding the same amount of sterile water. Add once a week, and after cultivating for one month, the incidence of tomato disease is counted. Such as image 3 As shown, the incidence of bacterial ...
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