Method for constructing Alzheimer disease mouse model

An Alzheimer's disease and mouse model technology, applied in the field of biomedicine, can solve problems such as being unsuitable for large-scale drug screening, the model making cycle is too long, and the spread of tau pathology cannot be observed.
CN110679549AActive Publication Date: 2020-01-14NANTONG UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANTONG UNIVERSITY
Publication Date
2020-01-14

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Abstract

The invention discloses a method for constructing an Alzheimer disease mouse model. A genotype FVB-Fgf14Tg (tetO-MAPT* P301L) 4510 Kha / JlwsJ female mouse is adopted, a truncated tau protein or a vector expressing the truncated tau protein is injected into the brain of the mouse at the age of 4 months and then the mouse is fed for 2-4 months, and the amino acid sequence of the truncated tau proteinis as shown in SEQ ID NO: I. The Alzheimer disease mouse model is successfully constructed, tau pathology only appears in the hippocampus region of the constructed mouse model, and does not appear inother brain regions, the construction period is shortened to 6 months, and more than 50% of modeling time is saved.
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Description

technical field

[0001] The invention belongs to the field of biomedicine, and in particular relates to a method for constructing an Alzheimer's disease mouse model Background technique

[0002] Alzheimer's Disease (AD for short), commonly known as senile dementia, is a common neurodegenerative disease in the elderly, manifested as learning and memory impairment, aphasia, apraxia, agnosia, and personality and behavior changes. dementia. AD has two major neuropathological features: extracellular amyloid plaques (amyloid plaques) formed by the accumulation of β-amyloid (Aβ) and intracellular neuronal neurons mainly formed by abnormally hyperphosphorylated tau protein. Fibrous tangle (Neurofibrillary tangle, NFT). So far, all clinical trials targeting Aβ have shown no significant effect (Iqbal, K., Liu, F., Gong, C.X., 2018. Recent developments with tau-based drug discovery. Expert Opin Drug Discov 13, 399-410.). More and more studies suggest that the spread of tau pathology ...

Claims

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