Combined use method of test strip and quantitative detection

Abstract

The invention discloses a combined use method of a test strip and quantitative detection. The method comprises the steps of firstly determining the concentration of a sample by using the test strip before quantitative detection, correspondingly judging the concentration range of the sample according to a color development condition of the test strip, and then determining the dilution factor of thesample needing to be diluted according to the color development condition of the test strip; then diluting the sample and then performing on-machine quantitative detection, thereby avoiding the problem that the repeated on-machine detection needs to be performed after diluting after the sample is directly and quantitatively detected on-machine, and avoiding the problems of high reagent cost and long detection period due to repeated on-machine quantitative detection. A detection report can be sent out after one-time quantitative detection, the detection period is obviously shortened, the detection efficiency is improved, the working time of laboratory personnel is shortened, the detection cost is saved, and meanwhile, the problem that an error report sheet is sent out due to hook-like effect when diluting the sample can be avoided.

Description

technical field [0001] The invention relates to the technical field of clinical detection, and more specifically relates to a method for combined use of test strips and quantitative detection. Background technique [0002] Antigen-antibody reaction is the most common detection method in clinical immunology. However, limited by the detection method, it is prone to bleeding in high-concentration samples. The hook effect refers to false negatives caused by inappropriate antigen-antibody ratios. This phenomenon can be recognized in the detection method of colloidal gold test strips, but it is often not recognized in quantitative detection. [0003] Immunocolloidal gold technology is a new type of immune labeling technology that uses colloidal gold as a tracer marker to apply to antigens and antibodies. The colloidal gold test strip detection method has the characteristics of short reaction time and low cost. Generally, it can be detected within 1 minute. However, due to the lac...

AI Technical Summary

Problems solved by technology

[0002] Antigen-antibody reaction is the most common detection method in clinical immunology. However, limited by the detection method, it is prone to bleeding in high-concentration samples. The hook effect refers to false negatives caused by inappropriate antigen-antibody ratios. This phenomenon can be identified in the colloidal gold test strip detection method, but it is often not recognized in the quantitative detection

[0003] Immunocolloidal gold technology is a new type of immune labeling technology that uses colloidal gold as a tracer marker to apply to antigens and antibodies. The colloidal gold test strip detection method has the characteristics of short reaction time and low cost. Generally, it can be detected within 1 minute. The results are measured, but due to the lack of sensitivity of colloidal gold and the inability to quantify, its clinical application is relatively limited. At present, it is only used for primary screening, and its daily use has gradually been replaced by other quantitative detection methods

[0004] At present, laboratories that use quantitative testing generally choose different dilution factors based on clinical diagnosis and then carry out testing on the machine. However, most laboratory application forms have incomplete information, which makes it impossible to determine the dilution factor. After a single test on the machine, it is easy to produce results If the detection limit is exceeded, it needs to be diluted and tested on the machine again, which is time-consuming (generally the detection cycle is 20-60 minutes), wastes cost, and it is difficult to find the hook effect, resulting in the issuance of false reports

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