Thoracoabdominal aortic aneurysm mouse model preparation method

A technology of aortic aneurysm and mouse model, applied in the field of biology, can solve the problems that the mechanism and process are not completely consistent, and the process of aortic aneurysm development cannot be well explored, and achieve the effect of simple operation and high tumor formation rate

Inactive Publication Date: 2020-01-17
ZHONGSHAN HOSPITAL FUDAN UNIV
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Problems solved by technology

[0004] The current methods are not completely consistent with the mechanism of aortic

Method used

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  • Thoracoabdominal aortic aneurysm mouse model preparation method
  • Thoracoabdominal aortic aneurysm mouse model preparation method
  • Thoracoabdominal aortic aneurysm mouse model preparation method

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Embodiment 1

[0017] Such as figure 1 Shown, the present invention provides a kind of preparation method of thoracoabdominal aortic aneurysm mouse model, comprises the steps:

[0018] 1) Select C57BL / 6 mice aged 9-11 weeks, and administer β-aminopropionitrile beta-aminopropionitrile (BAPN) (Energy Chemical, Shanghai, China) at an amount of 150 mg / kg / day to the animals for osmotic pressure The osmotic pump (Alzet, Cupertino, CA, USA) was continuously pumped subcutaneously for a total of 2 weeks;

[0019] 2) At the same time, angiotensin II (ApexBio, Boston, MA, USA) was continuously pumped subcutaneously through an osmotic pump (Alzet, Cupertino, CA, USA) at an amount of 1000 ng / kg / min. After 6 weeks, a mouse model of thoracoabdominal aortic aneurysm was obtained.

[0020] Specifically, the animal micro-administration osmotic pressure pump is subcutaneously implanted from the back of the mouse.

[0021] Such as figure 2 As shown, the mice that were injected with the drug for 6 weeks wer...

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Abstract

The invention provides a thoracoabdominal aortic aneurysm mouse model preparation method. The method includes: selecting a C57BL/6 mouse at the age of 9-11 weeks, and subcutaneously and continuously pumping BAPN (beta-aminopropionitrile) by 150mg/kg/day through an animal trace dosage osmotic pump for two weeks; subcutaneously and continuously pumping angiotensin II by 1000ng/kg/min through an animal trace dosage osmotic pump for six weeks to obtain a thoracoabdominal aortic aneurysm mouse model. By a beta-aminopropionitrile and angiotensin II combined method for inducing formation of thoracoabdominal aortic aneurysm of the C57BL/6 mouse, the established model better accords with a physiopathologic mechanism of thoracoabdominal aortic aneurysm formation, and further study on an aortic aneurysm pathological mechanism is benefited.

Description

technical field [0001] The invention belongs to the field of biology, and relates to an animal model, in particular to a method for preparing a thoracoabdominal aortic aneurysm mouse model. Background technique [0002] With the further research on the pathophysiological mechanism of aortic aneurysm, its inflammation and smooth muscle cell apoptosis lead to the decrease of the stability of the elastic membrane, the decrease of the elasticity and tension of the vessel wall, which leads to the expansion of the vessel wall and the formation of aneurysm. [0003] At present, there are mainly three methods for establishing aneurysm models. The mouse angiotensin Ⅱ induction method (mainly using ApoE- / - mice + high-fat diet) has a low rate of aneurysm formation, and aneurysms are often found in porridge. Plaque-like plaques appeared gradually after the formation, and the period was long, and the tumor-forming sites were almost all abdominal aorta. Elastase perfusion (complicated a...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02A01K2207/10A01K2207/20A01K2227/105A01K2267/0331
Inventor 朱铠赖颢胡诚凯李军程蕾蕾王春生
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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