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Use of antigen combination in preparation of kit for detecting related autoantibodies of lung cancer, and corresponding kit and detection method

An autoantibody and detection method technology, applied in kits and detection, antigen combination in the field of preparing kits for lung cancer-related autoantibody detection, can solve high false positives, insufficient detection sensitivity, and increase the workload of follow-up monitoring in the later period and economic burden to achieve reliable results

Inactive Publication Date: 2020-01-21
GENE TECH SHANGHAI COMPANY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, some technologies have used ELISA to simultaneously detect multiple autoantibodies for auxiliary diagnosis of lung cancer, for example, 6 (p53, NY-ESO-1, CAGE, GBU4-5, SOX2, Annexin I) and 7 (p53 , NY-ESO-1, CAGE, GBU4-5, MAGE-A4, SOX2, Hu-D) antigens were combined for detection, but due to the differences between Asians and European and American races, these antigen combinations are not suitable for Asian For the early detection of lung cancer in the population, the detection sensitivity is less than 30%. Another example is the combination of seven antigens, P53, PGP9.5, SOX2, GAGE ​​7, GBU4-5, MAGE A1 and CAGE. The sensitivity can reach 50%, but the sensitivity is low (about 30%) in the detection of early lung cancer, and there is also the problem of low specificity (about 80%). Higher false positives will further increase the work of follow-up monitoring Quantity and Economic Burden

Method used

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  • Use of antigen combination in preparation of kit for detecting related autoantibodies of lung cancer, and corresponding kit and detection method
  • Use of antigen combination in preparation of kit for detecting related autoantibodies of lung cancer, and corresponding kit and detection method
  • Use of antigen combination in preparation of kit for detecting related autoantibodies of lung cancer, and corresponding kit and detection method

Examples

Experimental program
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Embodiment 1

[0053] This example specifically illustrates the kit for combined detection of lung cancer-related autoantibodies that can be used to assist in the early screening and diagnosis of lung cancer and the corresponding detection method.

[0054] The kit provided in this example is prepared according to the principle of indirect enzyme-linked immunoassay, specifically: the antigenic protein corresponding to the autoantibody related to lung cancer is connected to the solid-phase carrier, and the autoantibody in the blood sample is combined with it to form a solid-phase carrier during detection. Phase antigen-test autoantibody complex, and then use enzyme-labeled secondary antibody (enzyme-labeled antibody) to combine with the autoantibody in the solid-phase antigen-test autoantibody complex to form a solid-phase antigen-test autoantibody-enzyme label The secondary antibody complex, and then measure the absorbance after adding the substrate to determine the level of the autoantibody t...

Embodiment 2

[0119] In this embodiment, serum samples of 148 early lung cancer patients (lung cancer group) and 142 healthy people (control group) were collected. All patients with lung cancer were diagnosed with early stage lung cancer (in situ, stage I and stage II) by CT examination with nodules less than 20mm. The serum of lung cancer patients was collected before receiving any radiotherapy and chemotherapy. The serum of healthy people came from healthy people without any malignant tumor-related diseases.

[0120] The kit and detection method of Example 1 were used to detect the levels of lung cancer-related autoantibodies in the sera of 148 lung cancer patients and 142 healthy people, respectively, and the detection results of the lung cancer-related autoantibodies in the control group and the lung cancer group were respectively obtained. Use simulated annealing to explore the cut-off value (Cut-off value) of each lung cancer-related autoantibody level. If it is higher than this value...

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Abstract

The invention provides a use of an antigen combination in the preparation of a kit for detecting related autoantibodies of lung cancer, and a corresponding kit and a detection method, in order to assist the early screening and diagnosis of the lung cancer more accurately. The use is characterized in that the antigen combination comprises the combinations of any seven or more of the following antigen proteins: P53, SOX2, GBU4-5, KRAS, P53-29, CK8, HuD, SSX1, P62, EGFR- VIII, P16, CK20, CAGE and NY-ESO-1.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the use of an antigen combination in preparing a kit for detecting lung cancer-related autoantibodies, as well as the corresponding kit and detection method. Background technique [0002] In the early stage of tumor development, tumor cells produce gene mutation, translocation or recombination, and after the release of related proteins, they are recognized by the immune system as "non-self proteins" as antigens (TAA) and an immune response occurs, producing antibodies against the antigen, namely Tumor autoantibodies (TAB). Autoantibodies circulate in the body in the form of free or combined with related antigens to form antigen-antibody complexes. The use of autoantibodies in the early diagnosis of cancer, efficacy evaluation, and prediction of recurrence has many advantages: autoantibodies can be detected in the asymptomatic stage of cancer; the serum of cancer patients ...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/68
CPCG01N33/57423G01N33/57488G01N33/6854
Inventor 刘浩王东李宾张涛
Owner GENE TECH SHANGHAI COMPANY