Anti-hair loss and hair growth composition
A technology for preventing hair loss and growth, composition, applied in the directions of drug combination, hair care, plant raw materials, etc., can solve the problems of side effects, drug dependence, and high surgical treatment costs
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Embodiment 1
[0031] Example 1 : Inhibition of 5α-reductase activity by birch sap stock solution and concentrated birch sap
[0032] In this example, the effects of birch sap stock solution and concentrated birch sap with different concentration ratios on the activity of 5α-reductase were investigated and compared.
[0033] 1. Concentration of birch sap
[0034] The fresh birch sap stock solution purchased from Daxinganling Chaoyue Wild Berry Development Co., Ltd. was input into a low-temperature drying equipment, cooled to -65°C, vacuumed to 0.1Pa, and concentrated to 1.2 times, 1.5 times, 2 times, 4 times, and 8 times respectively. times, 10 times.
[0035] 2. Test
[0036] Experimental equipment: balance, wall breaking machine, constant temperature oscillator, high-speed refrigerated centrifuge, microplate reader.
[0037] Experimental reagents and consumables:
[0038] Finasteride: Shanghai Modern Pharmaceutical Co., Ltd. (tablet 5mg / tablet);
[0039] Reduced coenzyme Ⅱ (NADPH): A...
Embodiment 2
[0059] Example 2 : Effects of birch sap stock solution and concentrated birch sap on the expression of genes and proteins related to anti-hair loss
[0060] In this example, the effects of birch sap stock solution and concentrated birch sap with different concentration ratios on the expression of genes and proteins related to anti-hair loss were investigated and compared.
[0061] 1. Concentration of birch sap
[0062] The fresh birch sap stock solution purchased from Daxinganling Chaoyue Wild Berry Development Co., Ltd. was input into a low-temperature drying equipment, cooled to -65°C, vacuumed to 0.1Pa, and concentrated to 1.2 times, 1.5 times, 2 times, 4 times, and 8 times respectively. times, 10 times.
[0063] 2. Test
[0064] The dermal papilla cells used in this experiment were produced by Guangdong Boxi Biotechnology Co., Ltd.
[0065] Experimental instrument: CO 2 Incubator (Thermo), Clean Bench (Sujing Antai), Inverted Microscope (Olympus), Micro Shaker (Qilin...
Embodiment 3
[0084] Example 3 : Effects of birch sap stock solution and concentrated birch sap on proliferation and differentiation of dermal papilla cells
[0085] In this example, the effects of birch sap stock solution and birch sap with different concentration ratios on the proliferation and differentiation of dermal papilla cells were investigated and compared.
[0086] 1. Concentration of birch sap
[0087] The fresh birch sap stock solution purchased from Daxinganling Chaoyue Wild Berry Development Co., Ltd. was input into a low-temperature drying equipment, cooled to -65°C, vacuumed to 0.1Pa, and concentrated to 1.2 times, 1.5 times, 2 times, 4 times, and 8 times respectively. times, 10 times.
[0088] 2. Test method
[0089] The dermal papilla cells used in this experiment were produced by Guangdong Boxi Biotechnology Co., Ltd.
[0090] Experimental instrument: CO 2 Incubator (Thermo), ultra-clean bench (Sujing Antai), inverted microscope (Olympus), micro shaker (Qilin Bell)...
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