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Process flow for cell continuous culture tank

A technology of process flow and culture tank, which is applied in the field of cell culture, can solve the problems of affecting growth and germination, and the decrease of enzyme activity, so as to achieve the effect of enhancing cell activity

Inactive Publication Date: 2020-01-31
苏州禾能生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, after the rice endosperm cells are cultured, the enzyme activity will decrease, thus affecting the growth and germination, and it is necessary to further improve the rice endosperm cell culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A kind of technological process of continuous cell culture tank of the present embodiment, comprises the following steps:

[0024] Step 1, pretreatment of rice endosperm cells: place the rice endosperm cells in an incubator for 20 minutes, the temperature of the incubator is 32°C, and the humidity is 70%, and then the modified pumpkin vine leaves are placed in the incubator, and then ultraviolet light is used to Irradiation treatment can be;

[0025] Step 2, activation of rice endosperm cells: place the pretreated rice endosperm cells into the activation nutrient solution for activation, the activation temperature is 35°C, and the activation time is 20 minutes, then take them out and put them in the culture medium for 10 minutes;

[0026] Step 3, culturing in a culture tank: send the rice endosperm cells into the culture tank for culture, the initial culture temperature is 34°C, culture for 10 minutes, and the culture period is 38°C, culture for 2 hours;

[0027] Step ...

Embodiment 2

[0035] A kind of technological process of continuous cell culture tank of the present embodiment, comprises the following steps:

[0036] Step 1, pretreatment of rice endosperm cells: place the rice endosperm cells in an incubator for 30 minutes, the temperature of the incubator is 38°C, and the humidity is 80%, then place the modified pumpkin vine leaves in the incubator, and then use ultraviolet Irradiation treatment can be;

[0037] Step 2, activation of rice endosperm cells: place the pretreated rice endosperm cells into the activation nutrient solution for activation, the activation temperature is 45°C, and the activation time is 30 minutes, then take them out and send them to the culture medium for 20 minutes;

[0038] Step 3, culturing in a culture tank: send the rice endosperm cells into the culture tank for culture, the initial temperature of the culture is 38°C, culture for 20min, and the later stage of culture is 42°C, culture for 5h;

[0039] Step 4, electromagnet...

Embodiment 3

[0046] A kind of technological process of continuous cell culture tank of the present embodiment, comprises the following steps:

[0047]Step 1, pretreatment of rice endosperm cells: place the rice endosperm cells in an incubator for 25 minutes. The temperature of the incubator is 35°C and the humidity is 75%. Irradiation treatment can be;

[0048] Step 2, activation of rice endosperm cells: place the pretreated rice endosperm cells into the activation nutrient solution for activation, the activation temperature is 40°C, and the activation time is 25 minutes, then take them out and send them to the culture medium for 5 minutes;

[0049] Step 3, culturing in a culture tank: send the rice endosperm cells into the culture tank for culture, the initial culture temperature is 36°C, cultivate for 15 minutes, and cultivate at 40°C for 3.5 hours in the later stage of culture;

[0050] Step 4, electromagnetic field cultivation: apply an electromagnetic field to the rice endosperm cell...

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Abstract

The invention discloses a process flow for a cell continuous culture tank. The process flow comprises the following steps of step I, pretreating rice endosperm cells: placing the rice endosperm cellsin a thermotank for standing at the temperature of 32-38 DEG C and at the humidity of 70-80% for 20-30min, then placing modified cushaw stems and leaves in the thermotank, and performing ultraviolet irradiation treatment. The process flow comprises the steps of pretreating the rice endosperm cells, activating the rice endosperm cells, subsequently performing culturing in the culture tank, and finally, performing culturing in an electromagnetic field, wherein pretreatment of the rice endosperm cells in the thermotank breaks dormancy of the rice endosperm cells, and the rice endosperm cells andthe modified cushaw stems and leaves are subjected to ultraviolet irradiation treatment, so that on one hand, the sterilization effect is achieved, on the other hand, the modified cushaw stems and leaves are frozen to preserve nutrient substances of a basal body, and through ultraviolet irradiation, the nutrient substances act on the rice endosperm cells.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a process flow of a continuous cell culture tank. Background technique [0002] Rice is the finished product of paddy after cleaning, husking, rice milling, finishing and other processes. The germ and aleurone layer of rice contain nearly 64% of rice nutrition and more than 90% of the nutrients needed by the human body. It is the main food of the southern people; soybean is one of the important food crops in China and has been cultivated for 5,000 years History, known as Shu in ancient times, is the main production area in Northeast China, and it is a crop whose seeds are rich in plant protein. [0003] In the prior art, the enzyme activity of rice endosperm cells will decrease after being cultured, thus affecting growth and germination, and it is necessary to further improve the culture of rice endosperm cells. Contents of the invention [0004] The object of the presen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04C12N13/00
CPCC12N5/04C12N13/00
Inventor 顾霆
Owner 苏州禾能生物科技有限公司
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