Debranching enzyme modified starch and preparing method and use thereof in hard capsule production
The technology of starch glue and hollow capsule is applied in the field of modified starch, which can solve the problems of undisclosed debranching enzymes and low degree of branching, and achieve the effect of strong and tough material and good film-forming property.
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Embodiment 1
[0023] Embodiment one, prepare the modified starch glue solution modified by isoamylase
[0024] Weigh 100 grams of starch, fully dissolve it in 500 grams of 85°C pure water, and then lower the temperature of the starch solution to 40-60°C. Add 0.1U-20000U of isoamylase to the resulting starch solution, and react at 40-60°C for 2 hours. Heat the reaction mixture to high temperature (above about 85°C) to destroy the enzyme and terminate the reaction. The temperature of the reaction mixture is lowered to 40-60° C. to complete the modified starch glue (1).
Embodiment 2
[0025] Embodiment two, prepare the modified starch glue solution modified by pullulanase
[0026] Weigh 100 grams of starch, fully dissolve it in 500 grams of 85°C pure water, and then lower the temperature of the starch solution to 40-60°C. 0.1U-20000U of pullulanase is added to the resulting starch solution and reacted at 40-60°C for 2 hours. Heat the reaction mixture to high temperature (above about 85°C) to destroy the enzyme and terminate the reaction. The temperature of the reaction mixture is lowered to 40-60° C. to complete the modified starch glue solution (2).
Embodiment 3
[0027] Embodiment three, prepare the modified starch glue modified by pullulanase
[0028] Weigh 100 grams of starch, fully dissolve it in 500 grams of 85°C pure water, and then lower the temperature of the starch solution to 40-60°C. 0.1U-20000U of pullulanase is added to the resulting starch solution and reacted at 40-60°C for 2 hours. Heat the reaction mixture to high temperature (above about 85°C) to destroy the enzyme and terminate the reaction. The reaction mixture was cooled again to 40-60 °C. Then add carrageenan with a weight percentage of 0.05% to 5% and potassium chloride with a weight percentage of 0.01% to 5% to complete the modified starch glue solution (3).
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