Adipose tissue cryopreservation method

Abstract

The invention discloses an adipose tissue cryopreservation method which comprises the following steps: I, uniformly injecting an appropriate amount of a swelling anesthetic fluid subcutaneously at a sampling site by using a porous water injection needle, after satisfactory anesthesia, connecting a liposuction needle with a syringe, and directly extracting adipose tissue into the syringe through negative pressure formed by a syringe tube so as to obtain an adipose tissue mixture for later use; II, adding an appropriate amount of normal saline to the extracted adipose tissue mixture, uniformly mixing the components by turning the components upside down, allowing the components to stand to enable granular adipose to float on an upper layer, discarding a liquid on a lower layer, cleaning supernate to remove most blood components till the color is yellow and bright, putting the supernate into a temperature-controlled centrifuge, performing centrifugation, and collecting granular adipose tissue for later use; III, preparing a mixed liquid of a trehalose solution and oil as a cryopreservation liquid; and IV, mixing the granular adipose tissue with the cryopreservation liquid according toa volume ratio of 1:1, putting the mixture into a refrigerator of 4 DEG C, 2 hours later, moving the mixture into a refrigerator of -20 DEG C, 4 hours later, moving the mixture into a deep low-temperature refrigerator of -80 DEG C for refrigeration, and 12 hours later, moving the mixture into liquid nitrogen for cryopreservation. By adopting the cryopreservation method disclosed by the invention,the activity of cryopreserved adipose tissue can be maintained.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for cryopreserving adipose tissue. Background technique [0002] Autologous fat particles are an ideal soft tissue filling material; however, the low survival rate and high absorption rate of transplanted fat particles are the main problems that hinder the widespread development and application of fat transplantation in clinic. A small amount, repeated, multiple injections are currently the ideal way to improve the survival rate of fat. However, repeated liposuction not only increases the patient's pain, but also increases medical risks and costs. Therefore, extracting more fat at one time for subsequent transplantation can solve the above problems to a large extent and reduce the number of medical staff. workload. "One liposuction, multiple injections" relies on the improvement of in vitro fat cryopreservation technology. In previous studies, normal saline and di...

AI Technical Summary

Problems solved by technology

In previous studies, normal saline and dimethyl sulfoxide (DMSO) were mostly used as cryopreservation base solutions, but the cell viability of cells preserved in normal saline was low; and DMSO has certain toxicity and cannot be used for cryopreservation. Clinical fat cell reinfusion

[0003] At present, the existing frozen fat technology has certain damage to the viability of fat cells