Adipose tissue cryopreservation solution and preparation method thereof

Abstract

The invention discloses an adipose tissue cryopreservation solution which comprises a 0.35mol / L trehalose solution, a grease and superoxide dismutase, wherein the volume ratio of the trehalose solution to the grease is 1:(1-3); and the concentration of the superoxide dismutase is 400-800IU / mL. According to the adipose tissue cryopreservation solution disclosed by the invention, on one hand, the trehalose solution is used as a novel fat cell stabilizer, and the grease is a better solvent, and mechanical damage caused to cells by formation of ice crystals in the freezing and cooling process canbe eliminated; and on the other hand, addition of an antioxidant can not only inhibit production of reactive oxygen, but also directly remove reactive oxygen, or reduce production of reactive oxygen by directly acting on elements of antioxidant reaction procedures to improve the level of endogenous antioxidants or inhibiting expression of oxygenase. Intervention of the antioxidant can effectivelyimprove the vitality of cryopreserved human granule adipose tissue and prevent cells from forming excessive peroxides due to oxidative stress in the cooling process.

Description

technical field [0001] The invention relates to the field of biomedical technology, in particular to an adipose tissue cryopreservation solution and a preparation method thereof. Background technique [0002] Autologous fat particles are an ideal soft tissue filling material; however, the low survival rate and high absorption rate of transplanted fat particles are the main problems that hinder the widespread development and application of fat transplantation in clinic. A small amount, repeated, multiple injections are currently the ideal way to improve the survival rate of fat. However, repeated liposuction not only increases the pain of the patient, but also increases the medical risks and costs. Therefore, a lot of fat is extracted at one time and transplanted in batches, which can largely solve the above problems and reduce the workload. "One liposuction, multiple injections" relies on the improvement of in vitro fat cryopreservation technology. In previous studies, norm...

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Problems solved by technology

"One liposuction, multiple injections" relies on the improvement of in vitro fat cryopreservation technology. In previous studies, normal saline and dimethyl sulfoxide (DMSO) were mostly used as cryopreservation base fluids, but physiological Cell viability is low after saline preservation; DMSO has certain toxicity and cannot be used clinically

[0003] In addition, the current frozen fat technology has damage to the viability of fat cells. Scholar Shoshanni believes that the two main aspects of fat cell viability damage are in the process of freezing and rewarming, not in the length of freezing storage time