Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kits for Male Fertility Diagnosis

A kit and fertility technology, applied in the field of medical testing, can solve problems such as infertility, inability to accurately reflect male fertility status, lack of molecular diagnostic indicators, etc., to achieve rapid and accurate evaluation results

Active Publication Date: 2021-07-09
THE FIRST HOSPITAL OF HEBEI MEDICAL UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Routine semen indicators such as sperm concentration, semen volume, total number of sperm, sperm motility, and sperm morphology are the "gold standard" for evaluating male fertility, but studies have pointed out that conventional semen parameters cannot accurately reflect male fertility status, and about 15% of normal semen parameters are normal patient with infertility
Currently, infertility assessment lacks molecular diagnostic indicators, therefore, it is necessary to find new indicators to indicate male fertility

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kits for Male Fertility Diagnosis
  • Kits for Male Fertility Diagnosis
  • Kits for Male Fertility Diagnosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Clinical use method of kit for diagnosis of male fertility

[0031] 1. Collection of research objects:

[0032] The main subjects were couples undergoing assisted reproductive technology, and the inclusion criteria were as follows: ①Patients with basically normal semen parameters and feasible IVF technology for assisted conception (the total number of forward sperm motility ≥ 20×10 6 , the proportion of normal sperm morphology ≥ 1%); ② female tubal factor infertile couples. All patients gave informed consent.

[0033] 2. RNA extraction reverse transcription and quantitative PCR:

[0034] After 2-7 days of abstinence, the patient masturbated to collect semen, and the total RNA of the sperm was extracted using a kit (Invitrogen), dissolved in DEPC water, and the concentration and purity of the total RNA were determined.

[0035] The expression of miR-122 and internal control U6 was detected by qRT-PCR. Among them, the 25 μL reverse transcription system is shown in Tab...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a molecular marker miR‑122, the nucleotide sequence of which is shown in SEQ ID NO:1. Also disclosed is a diagnostic kit for male fertility, a reagent for determining the expression level of the molecular marker miR-122 in semen, through which the expression level of miR-122 indicates the fertilization rate and blastocyst formation rate . The present invention can judge the fertilization, embryo development and clinical pregnancy by detecting the expression of miR‑122 in semen, and provides a simple and easy-to-operate method with high sensitivity for judging male fertility, which can be used as a new method for evaluating male fertility. index.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a kit for male fertility diagnosis. Background technique [0002] Infertility is a special reproductive health defect, often causing serious impact on the family and society. The incidence rate of infertility in the world is as high as 15%. Among infertile patients, infertility caused by purely male factors accounts for 20%, and both male and female factors account for 30-40%. Accurately judging male fertility is a prerequisite for diagnosing infertility and rationally treating it. Routine semen indicators such as sperm concentration, semen volume, total number of sperm, sperm motility, and sperm morphology are the "gold standard" for evaluating male fertility, but studies have pointed out that conventional semen parameters cannot accurately reflect male fertility status, and about 15% of normal semen parameters are normal The patient suffers from infertility. Currently, infer...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/113
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 李俊杨雪梅刘元科宋亚曼刘芳
Owner THE FIRST HOSPITAL OF HEBEI MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com