Tetracycline hollow double-imprinted layer magnetic nanospheres as well as preparation method and application thereof

A magnetic nanosphere, tetracycline technology, applied in chemical instruments and methods, other chemical processes, alkali metal oxides/hydroxides, etc., can solve the problems of increasing bacterial resistance, time-consuming separation process, complex efficiency, etc. The effect of surface functionalization, uniform particle size, and increased mass transfer rate

Active Publication Date: 2020-02-18
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tetracyclines may increase bacterial resistance
Due to the convenient operation of magnetic separation, combining this technology with molecular imprinting technology solves the problems of time-consuming, complicated and low-efficiency separation compared with the previous non-magnetic MIPs

Method used

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  • Tetracycline hollow double-imprinted layer magnetic nanospheres as well as preparation method and application thereof
  • Tetracycline hollow double-imprinted layer magnetic nanospheres as well as preparation method and application thereof
  • Tetracycline hollow double-imprinted layer magnetic nanospheres as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for preparing tetracycline hollow double-imprinted layer magnetic nanospheres includes the following steps:

[0038] Step 1. Put 1.0g ferric chloride hexahydrate, 20mL secondary water, 5mL ammonia water, 1.05g ferric chloride tetrahydrate in a three-necked flask, and react at 25°C for 10 minutes. After the reaction, the reaction product is ultra-pure Wash with water and vacuum dry at 20°C and 0.02MPa for 3h. Such as figure 1 As shown, the particle size of the prepared small-diameter magnetic nanospheres is about 5 nm.

[0039] Step 2. Put 5mL concentrated ammonia water, 10mL ethanol and 10mL distilled water in a three-necked flask, stir mechanically for 5min at a rotation speed of 1000r / min, add 1.5mL TEOS and 15mL ethanol to the above solution at a rotation speed of 100r / min Under the condition of mechanical stirring for 1h, finally centrifuged to obtain silica nanospheres. Such as figure 2 As shown, the particle size of the prepared silica nanospheres is about ...

Embodiment 2

[0052] A method for preparing tetracycline hollow double-imprinted layer magnetic nanospheres includes the following steps:

[0053] Step 1. Put 2.0 g of ferric chloride hexahydrate, 30 mL of secondary water, 8 mL of ammonia, and 2.72 g of ferric chloride tetrahydrate in a three-necked flask, and react at 40°C for 50 minutes. After the reaction, the reaction product is ultra-pure Washing with water, vacuum drying at 30°C and 0.04 MPa for 4 hours, to prepare small particle size magnetic nanospheres.

[0054] Step 2. Place 10mL concentrated ammonia water, 21mL ethanol and 22mL distilled water in a three-necked flask, stir mechanically for 10min at a rotation speed of 1200r / min, add 3mL TEOS and 25mL ethanol to the above solution at a rotation speed of 150r / min Under mechanical stirring for 3 hours, and finally centrifuge to obtain silica nanospheres.

[0055] Step 3. Put 15mg magnetic nanospheres, 15mg tetracycline, 40mg silicon spheres, 60mg dopamine and 10mL Tris-HCl in the reaction...

Embodiment 3

[0067] A method for preparing tetracycline hollow double-imprinted layer magnetic nanospheres includes the following steps:

[0068] Step 1. Put 4.5g of ferric chloride hexahydrate, 40mL of secondary water, 12mL of ammonia water, and 3.5g of ferric chloride of tetrahydrate in a three-necked flask, and react at 55°C for 100 minutes. After the reaction is over, the reaction product is ultra-pure Wash with water and vacuum dry at 40°C and 0.05MPa for 5h. Prepared small particle size magnetic nanospheres.

[0069] Step 2. Put 15mL concentrated ammonia water, 30mL ethanol and 35mL distilled water in a three-necked flask, stir mechanically for 15min at a rotation speed of 1500r / min, add 5mL TEOS and 35mL ethanol to the above solution at a rotation speed of 200r / min Under mechanical stirring for 5 hours, and finally centrifuged to obtain silica nanospheres.

[0070] Step 3. Place 20mg magnetic nanospheres, 18mg tetracycline, 70mg silicon spheres, 78mg dopamine and 15mL Tris-HCl in the rea...

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Abstract

The invention discloses tetracycline hollow double-imprinted layer magnetic nanospheres as well as a preparation method and application thereof and belongs to the technical field of preparation of nanomaterials and application in environmental chemistry. The preparation method comprises the following steps: firstly, synthesizing magnetic nanoparticles with a small particle size by using a co-precipitation method at one step; synthesizing silicon spheres by using a method; further adding the silicon spheres, magnetic spheres, dopamine and tetracycline to a Tris-HCl buffer, performing sufficientstirring at room temperature, performing washing, separation and vacuum drying on the product, and repeating a wrapping step; and finally etching off the silicon spheres by using sodium carbonate soas to form a polymer of a hollow structure, separating out the generated polymer by using external magnetic spheres, and performing elution and vacuum drying, so as to obtain the tetracycline hollow double-imprinted layer magnetic nanospheres. The tetracycline hollow double-imprinted layer magnetic nanospheres disclosed by the invention are uniform in particle size, good in structure stability, strong in magnetic responsiveness, good in reusability, large in selective adsorption capacity for tetracycline, large in adsorption capacity, fast in mass transfer rate, high in recovery rate, meanwhile, low in material cost and simple to prepare.

Description

Technical field [0001] The invention belongs to the technical field of nano material preparation and environmental chemistry application, and specifically relates to a tetracycline hollow double imprinted layer magnetic nanosphere, and a preparation method and application thereof. Background technique [0002] Tetracycline antibiotics are antibacterial substances isolated from the culture broth of the actinomyces Streptomyces aureofa-ciens. They are resistant to Gram-positive bacteria, negative bacteria, rickettsiae, filtering viruses, spirochetes and even Protozoa have a good inhibitory effect and are a broad-spectrum antibiotic. Preparations obtained by chemically modifying natural tetracycline (pymetracycline, etc.) and semi-synthetic tetracycline derivatives (methadone, etc.) have been used in medical practice. Currently, there are about forty natural tetracyclines and more than 3,000 synthetic tetracyclines known. Tetracycline is a yellow crystalline substance, easily solu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/28B01J20/30C02F1/28C02F101/34
CPCB01J20/06B01J20/103B01J20/262B01J20/28009B01J20/28021B01J2220/46B01J2220/4806B01J2220/4812C02F1/281C02F1/285C02F2101/34
Inventor 高瑞霞王悦徐媛田雪蒙赵文昌
Owner XI AN JIAOTONG UNIV
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