Method for establishing insulin resistance H9c2 cell model by induction of sodium oleate

Abstract

The invention relates to a method for establishing an insulin resistance H9c2 cell model by induction of sodium oleate. The invention can effectively solve the problems that an existing insulin resistance cell model is difficult to establish, the modeling rate is low when high-concentration glucose is adopted to induce insulin resistance, and insulin has great instability and is very difficult tostore as a hormone substance. The technical scheme is as follows: 1) performing cell culture; 2) screening a sodium oleate working concentration by a glucose consumption experiment; and 3) carrying out a process of In-cell Western. According to the invention, sodium oleate is selected to induce rat myocardial cell strains, and the insulin resistance model is successfully established. The fact thatsodium oleate can induce cell insulin resistance is verified for the first time, an induction mechanism of sodium oleate is determined, a new application of sodium oleate is opened up, and the methodis an innovation in inducing establishment of an insulin resistance model by sodium oleate.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a method for establishing an insulin-resistant H9c2 cell model induced by sodium oleate. Background technique [0002] Insulin resistance is a pathological state in which the body's sensitivity to physiological levels of insulin decreases due to various reasons. It can be manifested as elevated fasting blood sugar, hyperinsulinemia, blood lipid disorders, hypertension, etc., and is the basis of type Ⅱ diabetes. , is also at the heart of the metabolic syndrome. A variety of metabolic diseases caused by insulin resistance will increase the morbidity and mortality of patients with myocardial injury. Finding active compounds for the treatment of insulin resistance and proving the mechanism of action of these compounds at the cellular level is the unshirkable responsibility of contemporary researchers. [0003] Currently commonly used methods for establishing insulin-resistant cell models in...

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Problems solved by technology

[0005] In view of the above situation, in order to solve the defects of the prior art, the object of the present invention is to provide a method for establishing a model of insulin-resistant H9c2 cells induced by sodium oleate, which can effectively solve the difficulty of modeling existing insulin-resistant cell models. When the concentration of glucose induces insulin resistance, the molding rate is low. As a hormone substance, insulin has great instability and is very difficult to preserve.

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