Method for establishing insulin resistance H9c2 cell model by induction of sodium oleate

An insulin resistance and cell model technology, applied in the field of medicine, can solve the problems of difficulty in modeling insulin resistance cell models, instability, and low molding rate.
CN110804583AInactive Publication Date: 2020-02-18HENAN UNIV OF CHINESE MEDICINE
0 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HENAN UNIV OF CHINESE MEDICINE
Publication Date
2020-02-18
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a method for establishing an insulin resistance H9c2 cell model by induction of sodium oleate. The invention can effectively solve the problems that an existing insulin resistance cell model is difficult to establish, the modeling rate is low when high-concentration glucose is adopted to induce insulin resistance, and insulin has great instability and is very difficult tostore as a hormone substance. The technical scheme is as follows: 1) performing cell culture; 2) screening a sodium oleate working concentration by a glucose consumption experiment; and 3) carrying out a process of In-cell Western. According to the invention, sodium oleate is selected to induce rat myocardial cell strains, and the insulin resistance model is successfully established. The fact thatsodium oleate can induce cell insulin resistance is verified for the first time, an induction mechanism of sodium oleate is determined, a new application of sodium oleate is opened up, and the methodis an innovation in inducing establishment of an insulin resistance model by sodium oleate.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of medicine, in particular to a method for establishing an insulin-resistant H9c2 cell model induced by sodium oleate. Background technique

[0002] Insulin resistance is a pathological state in which the body's sensitivity to physiological levels of insulin decreases due to various reasons. It can be manifested as elevated fasting blood sugar, hyperinsulinemia, blood lipid disorders, hypertension, etc., and is the basis of type Ⅱ diabetes. , is also at the heart of the metabolic syndrome. A variety of metabolic diseases caused by insulin resistance will increase the morbidity and mortality of patients with myocardial injury. Finding active compounds for the treatment of insulin resistance and proving the mechanism of action of these compounds at the cellular level is the unshirkable responsibility of contemporary researchers.

[0003] Currently commonly used methods for establishing insulin-resistant cell models in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More