A kind of detection device preparation method and detection device

Abstract

The embodiment of the invention relates to the field of biology, and discloses a method for preparing a detection device and a detection device. The detection device preparation method provided in the present invention includes: providing a detection chip and a well array located on the detection chip; the well array is composed of a plurality of wells; forming a first film layer on the side wall of the well through a self-alignment process, the first The material of the film layer is different from the material of the lower surface of the well, and the material of the first film layer is different from the material of the upper surface of the well array; the first film layer is used to modify specific substances on the side walls of the wells, and the specific substances are used for adsorption or binding molecule to be tested. The embodiment of the present invention can realize the precise modification of the side wall of the well, and can directly modify the molecules to be measured on the side wall of the well without relying on microspheres during biological detection, solving the problem of complex microsphere manufacturing process and the integration degree of semiconductor biochips. Inferior question.

Description

technical field [0001] The embodiments of the present invention relate to the field of biology, and in particular to a method for preparing a detection device and a detection device. Background technique [0002] At present, large-scale micro-nano-scale arrays are applied in the field of laboratory-on-a-chip. Its large-scale batch production, high efficiency of biochemical reactions, and high controllability of the environment have been favored by chemical and biological experts. Gene sequencing is a typical application of large-scale arrays. In gene sequencing, it is necessary to modify the surface of the array in order to adsorb adapters that can capture nucleic acid molecules to be tested. In the prior art, photolithography or printing techniques are usually used to modify the target region; the conventional sequencing method is to combine adapters, primers and DNA fragments are attached to microspheres, and the modified microspheres are then loaded into an array of micr...

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Problems solved by technology

[0005] The purpose of the embodiments of the present invention is to provide a detection device preparation method and a detection device, which can achieve precise modification of the side wall of the well, and can directly modify the molecules to be tested on the side wall of the well without relying on microspheres during biological detection , to solve the problems of complex microsphere manufacturing process and low integration of semiconductor biochips

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