Detection device preparation method and detection device

Abstract

The embodiment of the invention relates to the field of biology, and discloses a detection device preparation method and a detection device. The preparation method of the detection device provided bythe invention comprises the following steps: providing a detection chip and a well array located on the detection chip, wherein the well array is composed of a plurality of wells; forming a first filmlayer on the side walls of the wells through a self-alignment process, wherein the material of the first film layer is different from the material of the lower surfaces of the wells, and the materialof the first film layer is different from the material of the upper surface of the well array, and the first film layer is used for modifying a specific substance on the side walls of the wells, andthe specific substance is used for adsorbing or combining to-be-detected molecules. According to the embodiment of the invention, the side walls of the wells can be accurately modified, molecules to be detected can be directly modified on the side walls of the wells without depending on microspheres during biological detection, and the problems of complex microsphere manufacturing process, low semiconductor biochip integration level and the like are solved.

Description

technical field [0001] The embodiments of the present invention relate to the field of biology, and in particular to a method for preparing a detection device and a detection device. Background technique [0002] At present, large-scale micro-nano-scale arrays are applied in the field of laboratory-on-a-chip. Its large-scale batch production, high efficiency of biochemical reactions, and high controllability of the environment have been favored by chemical and biological experts. Gene sequencing is a typical application of large-scale arrays. In gene sequencing, it is necessary to modify the surface of the array in order to adsorb adapters that can capture nucleic acid molecules to be tested. In the prior art, photolithography or printing techniques are usually used to modify the target region; the conventional sequencing method is to combine adapters, primers and DNA fragments are attached to microspheres, and the modified microspheres are then loaded into an array of micr...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the embodiments of the present invention is to provide a detection device preparation method and a detection device, which can achieve precise modification of the side wall of the well, and can directly modify the molecules to be tested on the side wall of the well without relying on microspheres during biological detection , to solve the problems of complex microsphere manufacturing process and low integration of semiconductor biochips

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