Detection reagent for N-acetyl-beta-D-glucosidase

A technology of glucosamine and detection reagent, which is applied in the measurement of color/spectral characteristics, material analysis by observing the effect on chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of poor stability and sensitivity, etc. problem, to achieve the effect of improving strong alkaline environment, improving sensitivity and ensuring efficiency

Inactive Publication Date: 2020-02-28
吉林省富生医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current substrates generally have shortcomings such as poor stability and sensitivity.

Method used

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  • Detection reagent for N-acetyl-beta-D-glucosidase
  • Detection reagent for N-acetyl-beta-D-glucosidase
  • Detection reagent for N-acetyl-beta-D-glucosidase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A traditional PNP-NAG method detection reagent, the composition of described reagent R1 and reagent R2 is as follows:

[0031] R1 reagent:

[0032] Glycine buffer 30mmol / L

[0033] p-Nitrophenyl-β-D-glucosamine 16mmmol / L

[0034] Sodium azide 1g / L

[0035] R2 reagent:

[0036] Borax buffer at pH 10.2 100mmol / L

[0037] Sodium azide 1g / L

Embodiment 2

[0039] NAG detection reagent of the present invention comprises reagent R1 and reagent R2, and the composition of described reagent R1 and reagent R2 is as follows:

[0040] R1 reagent composition:

[0041]

[0042] R2 reagent composition:

[0043] Sodium carbonate buffer 10~100mmol / L

[0044] Triton X-100 3mL / L~5mL / L

[0045] Preservatives 2g / L~5g / L.

[0046] The citric acid buffer solution is a citric acid buffer solution with a pH of 4-5 at 25°C.

[0047] The stabilizer is selected from one of EDTA, EDTA-2Na, ethylene glycol, and iminodiacetic acid.

[0048] The preservative is selected from one of NaN3, methyl p-hydroxybenzoate and 4-chloro-3,5-dimethylphenol.

[0049] Respectively, the reagents obtained in Example 1 and Example 2 were detected and used in a fully automatic biochemical analyzer with dual reagent functions, and determined by the rate method.

[0050] Detection and use method: place reagents R1 and R2 on the corresponding reagent positions according...

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Abstract

The invention discloses a detection reagent for N-acetyl-beta-D-glucosidase. The detection reagent comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises 10-100mmol/L of citric acidbuffer solution, 0.1-0.3g/L of substrate, 1KU/L-5KU/L of ascorbic acid oxidase, 0.01-0.03g/L of magnesium chloride, 1-10g/L of stabilizer, and 2g/L-5g/L of preservative, the reagent R2 comprises 10-100mmol/L of sodium carbonate buffer solution, 3mL/L-5mL/L of triton X-100, and 2g/L-5g/L of preservative, the citric acid buffer solution is at 25 DEG C and the pH thereof is 4-5, the substrate is 5-[4-(3-methoxy-benzophene-rhodanine)-3-ammonium acetate-N-acetylamino-beta-D-glucoside, the stabilizer is one of EDTA, EDTA-2Na, glycol, and diglycolamidic acid, and the preservative is one of NaN3, methylparaben, and 4-chloro-3,5-dimethylphenol. A synthesized substrate 5-(4-(3-methyl-styrene)-rhodane-3-acetic acid amino-N-acetamino-beta-D-glucoside is used as a reaction substrate, the substrate isrelatively low-cost, and a reactant has high color development performance, so that the reagent is more sensitive.

Description

technical field [0001] The invention relates to a detection reagent for N-acetyl-β-D-glucosaminidase. Background technique [0002] N-acetyl-β-D-glucosaminidase (NAG for short) is a lysosomal enzyme, also known as urease. Widely distributed in various tissues of the human body, but the highest content in the prostate and proximal renal tubules of the kidney. The molecular weight of NAG is about 130-140KD. Under normal circumstances, NAG in serum cannot be excreted from urine through glomerular filtration. The increase of NAG in urine is an early manifestation of kidney disease and a sensitive indicator of renal tubular damage. For kidney transplant patients, urine NAG determination can detect early rejection. Generally, urinary NAG increases 1-3 days before clinical indications. In the late 1980s, the application research in the field of diabetic nephropathy continued to increase. It is worth noting that the increase of urinary NAG in the early stage of diabetic nephropat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/78G01N21/31
Inventor 张海悦徐岩程慧敏赵青周玉龙孙景春张国光
Owner 吉林省富生医疗器械有限公司
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