Application of ews or its upregulator in the preparation of medicines for treating diabetes and preventing and treating tumors in individuals with diabetes

A technology of diabetic retina and drug, applied in the field of biomedicine, to achieve the effect of increasing AMPK activity, reducing JNK activity and relieving dysfunction

Active Publication Date: 2022-03-15
SHANGHAI FIRST PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no literature has reported the role of EWS in the prevention and treatment of diabetic vascular complications and tumors

Method used

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  • Application of ews or its upregulator in the preparation of medicines for treating diabetes and preventing and treating tumors in individuals with diabetes
  • Application of ews or its upregulator in the preparation of medicines for treating diabetes and preventing and treating tumors in individuals with diabetes
  • Application of ews or its upregulator in the preparation of medicines for treating diabetes and preventing and treating tumors in individuals with diabetes

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Experimental program
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Effect test

Embodiment 1

[0081] 1. Method

[0082] 1. Viral vector construction for overexpressing EWS (named: E-OE) or Fbxw7 (named: F-OE) protein

[0083](1) Amplify the nucleotide sequences of EWS and Fbxw7 by PCR reaction (the sequence of EWS is shown in SEQ ID NO: 1, and the sequence of Fbxw7 is shown in SEQ ID NO: 2), and the two ends are connected with EcoRI and BamHI restriction sites point;

[0084] (2) double restriction digestion of PCR products and purification;

[0085] (3) Also double digest the H201pAdeno-MCMV-EGFP-3FLAG vector plasmid (Shanghai Heyuan Biotechnology Co., Ltd.), recover and purify the large fragment, and insert the EWS or Fbxw7 gene fragment in step (2) using molecular cloning technology instead of EGFP to the EcoRI and BamHI restriction sites of the shuttle plasmid;

[0086] (4) Colony PCR screens transformants, and the screened positive clones are sequenced and verified;

[0087] (5) Sequencing verifies the correct clones, and extracts high-purity plasmids;

[008...

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Abstract

The invention relates to the application of EWS or its upregulator in the preparation of medicines for treating diabetes and preventing and treating tumors in diabetic individuals. The present invention finds that EWS significantly reduces the high ROS level and excessive activation of PARP in diabetic macrovascular, microvascular endothelial and retinal tissues; Repairing damaged DNA not only alleviated the high glucose toxicity of vascular endothelium, but also showed retinal protection; EWS could significantly enhance the catabolism and AMPK activity of large vessel endothelial cells, and reduce the JNK activity. It is suggested that EWS or its up-regulator can be used to prepare drugs for alleviating the dysfunction of diabetic vascular endothelium, or strengthening DNA damage repair, or treating diabetes, or preventing diabetic complications, or preventing tumor occurrence in diabetic individuals.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of EWS or its upregulator in the preparation of medicines for treating diabetes and preventing and treating tumors in diabetic individuals. Background technique [0002] Diabetes mellitus (DM) is a lifelong metabolic disease characterized by chronic hyperglycemia. Diabetic vascular disease is a characteristic pathological change of diabetes and the main cause of disease and disability in patients. Long-term hyperglycemia can damage large blood vessels and microvessels, which in turn seriously endangers the heart, brain, kidneys, peripheral nerves, eyes, feet, etc. [1]. Statistics from the American Diabetes Association (American Diabetes Association, ADA) show that the probability of complications in patients with diabetes over 3 years is over 46%; the chance of complications in patients with diabetes over 5 years is over 61%; Patients have a 98% chance of de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61P35/00A61P3/10A61P25/00A61P9/14A61P9/10A61P13/12
CPCA61K45/00A61P35/00A61P3/10A61P25/00A61P9/14A61P9/10A61P13/12
Inventor 韦芳许迅邓骏杰姚谢怡顾青
Owner SHANGHAI FIRST PEOPLES HOSPITAL
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