Application of Ewing sarcoma (EWS) or up-regulator thereof in preparing medicament for treating diabetes and preventing and treating tumorigenesis of diabetic individuals

A technology for diabetes, diabetic blood vessels, applied in the field of biomedicine

Active Publication Date: 2020-03-10
SHANGHAI FIRST PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no literature has reported the role of EWS in the prevention and treatment of diabetic vascular complications and tumors

Method used

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  • Application of Ewing sarcoma (EWS) or up-regulator thereof in preparing medicament for treating diabetes and preventing and treating tumorigenesis of diabetic individuals
  • Application of Ewing sarcoma (EWS) or up-regulator thereof in preparing medicament for treating diabetes and preventing and treating tumorigenesis of diabetic individuals
  • Application of Ewing sarcoma (EWS) or up-regulator thereof in preparing medicament for treating diabetes and preventing and treating tumorigenesis of diabetic individuals

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Experimental program
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Embodiment 1

[0081] 1. Method

[0082] 1. Viral vector construction for overexpressing EWS (named: E-OE) or Fbxw7 (named: F-OE) protein

[0083](1) Amplify the nucleotide sequences of EWS and Fbxw7 by PCR reaction (the sequence of EWS is shown in SEQ ID NO: 1, and the sequence of Fbxw7 is shown in SEQ ID NO: 2), and the two ends are connected with EcoRI and BamHI restriction sites point;

[0084] (2) double restriction digestion of PCR products and purification;

[0085] (3) Also double digest the H201pAdeno-MCMV-EGFP-3FLAG vector plasmid (Shanghai Heyuan Biotechnology Co., Ltd.), recover and purify the large fragment, and insert the EWS or Fbxw7 gene fragment in step (2) using molecular cloning technology instead of EGFP to the EcoRI and BamHI restriction sites of the shuttle plasmid;

[0086] (4) Colony PCR screens transformants, and the screened positive clones are sequenced and verified;

[0087] (5) Sequencing verifies the correct clones, and extracts high-purity plasmids;

[008...

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Abstract

The invention relates to an application of Ewing sarcoma (EWS) or an up-regulator thereof in preparing a medicament for treating diabetes and preventing and treating tumorigenesis of diabetic individuals. The invention finds that the EWS obviously reduces the high ROS level and excessive activation of poly-(ADP-ribose)polymerase (PARP) in diabetic macrovascular and microvascular endothelia and retinal tissues; the EWS protein repairs damaged DNA by activating a homologous recombination repair (HR) pathway and a non-homology end joining (NHEJ) pathway, so that high-sugar toxicity of the vascular endothelia is relieved, and a retinal protection effect is shown; and the EWS can obviously enhance catabolism and AMPK activity of macrovascular endothelial cells and reduce JNK activity. It suggests that EWS or the up-regulator thereof can be used for preparing medicines for relieving dysfunction of diabetic vascular endothelia, or strengthening repair of DNA damage, or treating diabetes, or preventing and treating diabetic complications, or for preventing and treating tumorigenesis of diabetic individuals.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of EWS or its upregulator in the preparation of medicines for treating diabetes and preventing and treating tumors in diabetic individuals. Background technique [0002] Diabetes mellitus (DM) is a lifelong metabolic disease characterized by chronic hyperglycemia. Diabetic vascular disease is a characteristic pathological change of diabetes and the main cause of disease and disability in patients. Long-term hyperglycemia can damage large blood vessels and microvessels, which in turn seriously endangers the heart, brain, kidneys, peripheral nerves, eyes, feet, etc. [1]. Statistics from the American Diabetes Association (American Diabetes Association, ADA) show that the probability of complications in patients with diabetes over 3 years is over 46%; the chance of complications in patients with diabetes over 5 years is over 61%; Patients have a 98% chance of de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61P35/00A61P3/10A61P25/00A61P9/14A61P9/10A61P13/12
CPCA61K45/00A61P35/00A61P3/10A61P25/00A61P9/14A61P9/10A61P13/12
Inventor 韦芳许迅邓骏杰姚谢怡顾青
Owner SHANGHAI FIRST PEOPLES HOSPITAL
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