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Application of fbxw7 or its upregulator in the preparation of medicines for treating diabetes and preventing and treating tumors in diabetic individuals

A diabetic retina and drug technology, applied in the field of biomedicine, can solve the problem that the obese mouse model is not equivalent to diabetes, etc., and achieve the effects of strengthening DNA damage repair, reducing insulin resistance, and reducing JNK activity

Active Publication Date: 2022-03-15
SHANGHAI FIRST PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the study showed that the overexpression of FBXW7 in the liver of obese mouse models prevents insulin resistance, lowers blood sugar, and prevents obesity from developing into diabetes, the obese mouse model is not equivalent to hyperglycemia or diabetes. So far, no literature has reported the role of FBXW7 in the prevention and treatment of diabetic vascular complications and tumors

Method used

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  • Application of fbxw7 or its upregulator in the preparation of medicines for treating diabetes and preventing and treating tumors in diabetic individuals
  • Application of fbxw7 or its upregulator in the preparation of medicines for treating diabetes and preventing and treating tumors in diabetic individuals
  • Application of fbxw7 or its upregulator in the preparation of medicines for treating diabetes and preventing and treating tumors in diabetic individuals

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Experimental program
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Effect test

Embodiment 1

[0081] 1. Method

[0082] 1. Viral vector construction for overexpressing EWS (named: E-OE) or Fbxw7 (named: F-OE) protein

[0083] (1) Amplify the nucleotide sequences of EWS and Fbxw7 by PCR reaction (the sequence of EWS is shown in SEQ ID NO: 1, and the sequence of Fbxw7 is shown in SEQ ID NO: 2), and the two ends are connected with EcoRI and BamHI restriction sites point;

[0084] (2) double restriction digestion of PCR products and purification;

[0085] (3) Also double digest the H201pAdeno-MCMV-EGFP-3FLAG vector plasmid (Shanghai Heyuan Biotechnology Co., Ltd.), recover and purify the large fragment, and insert the EWS or Fbxw7 gene fragment in step (2) using molecular cloning technology instead of EGFP to the EcoRI and BamHI restriction sites of the shuttle plasmid;

[0086] (4) Colony PCR screens transformants, and the screened positive clones are sequenced and verified;

[0087] (5) Sequencing verifies the correct clones, and extracts high-purity plasmids;

[00...

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Abstract

The invention relates to the application of FBXW7 or its upregulator in the preparation of medicines for treating diabetes and preventing and treating tumors in diabetic individuals. The present invention finds that FBXW7 significantly reduces high ROS levels and excessive activation of PARP in diabetic macrovascular, microvascular endothelial and retinal tissues; Repairing damaged DNA not only alleviates the high glucose toxicity of vascular endothelium, but also exhibits retinal protection; FBXW7 can significantly enhance the catabolism and AMPK activity of large vessel endothelial cells, and reduce the JNK activity. It is suggested that FBXW7 or its upregulator can be used to prepare drugs for alleviating the dysfunction of diabetic vascular endothelium, or strengthening DNA damage repair, or treating diabetes, or preventing diabetic complications, or preventing tumor occurrence in diabetic individuals.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of FBXW7 or its upregulator in the preparation of medicines for treating diabetes and preventing and treating tumors in diabetic individuals. Background technique [0002] Diabetes mellitus (DM) is a lifelong metabolic disease characterized by chronic hyperglycemia. Diabetic vascular disease is a characteristic pathological change of diabetes and the main cause of disease and disability in patients. Long-term hyperglycemia can damage large blood vessels and microvessels, which in turn seriously endangers the heart, brain, kidneys, peripheral nerves, eyes, feet, etc. [1]. Statistics from the American Diabetes Association (American Diabetes Association, ADA) show that the probability of complications in patients with diabetes over 3 years is over 46%; the chance of complications in patients with diabetes over 5 years is over 61%; Patients have a 98% chance of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61P3/10A61P9/10A61P13/12A61P35/00
CPCA61K45/00A61P3/10A61P9/10A61P13/12A61P35/00
Inventor 韦芳许迅邓骏杰姚谢怡顾青
Owner SHANGHAI FIRST PEOPLES HOSPITAL
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