Reagent for identifying African swine fever gene deleted vaccine and detection method and application thereof

Abstract

The invention discloses a reagent for identifying an African swine fever gene deleted vaccine and a detection method and application thereof. The reagent comprises three primer-probe compositions, a forward primer, a reverse primer and a probe of the first primer-probe composition are shown in the formulas of Seq ID No. 1-3, a forward primer, a reverse primer and a probe of the second primer-probecomposition are shown in the formulas of Seq ID No.4-6, forward and reverse primers and a probe of the third primer-probe composition are shown in the formulas of s Seq ID No. 7-9, ends 5' of the three probes are modified with different fluorophores, and tail ends 3' of the three probes are modified with fluorescence quenching groups. According to the reagent disclosed by the invention, differentgene deleted vaccines can be identified in the same reaction by adopting the three primer-probe compositions, a specific and sensitive detection reagent is provided for rapid identification of African swine fever vaccines, a powerful scientific tool is provided for preventing African swine fever virus propagation, and the reagent has important significance for effective prevention and control ofAfrican swine fever epidemic situations.

Description

technical field [0001] The application relates to the field of African swine fever vaccine detection, in particular to a reagent, detection method and application for identifying African swine fever gene deletion vaccine. Background technique [0002] African swine fever (African swine fever, ASF) is caused by African swine fever virus (ASF virus, ASFV), an acute, severe, highly contagious zoonotic disease of domestic pigs and wild boars, all breeds and ages of pigs can be Infection, morbidity and mortality can reach up to 100%. Although African swine fever is not a zoonotic disease, the disease is extremely harmful to the pig industry. Once an epidemic occurs, it is difficult to eradicate it in a short period of time. It is a disease that the pig industry worldwide focuses on preventing. The World Organization for Animal Health (OIE) lists African swine fever as a legally notifiable disease, and my country lists it as a first-class animal disease. [0003] It has been nea...

AI Technical Summary

Problems solved by technology

[0006] Although there is currently no African swine fever vaccine approved for marketing at home and abroad; however, current research shows that the African swine fever gene deletion vaccine is the most likely and most promising African swine fever vaccine

Therefore, in the foreseeable future, how to effectively distinguish the African swine fever gene deletion vaccine from the wild strain infected with the disease will seriously interfere with the prevention and control of African swine fever

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